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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB, IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB10910 描述 Anti-Osteopontin Antibody Alternate Names - secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
- Bone sialoprotein 1
- SPP1/CALPHA1 fusion
- Urinary stone protein
- bone sialoprotein I
- osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein
- secreted phosphoprotein 1
- secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
- bone sialoprotein I
- early T-lymphocyte activation 1)
- secreted phosphoprotein-1 (osteopontin
背景信息 Osteopontin is a single-chain polypeptide. It is highly phosphorylated sialoprotein that is a prominent component of the mineralized extracellular matrices of bones and teeth. It is characterized by the presence of a polyaspartic acid sequence and sites of Ser/Thr phosphorylation that mediate hydroxyapatite binding, and a highly conserved RGD motif that mediates cell attachment/signaling. Expression of osteopontin in a variety of tissues indicates a multiplicity of functions that involve one or more of these conserved motifs. While the lack of a clear phenotype in osteopontin "knockout" mice has not established a definitive role for osteopontin in any tissue, recent studies have provided some novel and intriguing insights into the versatility of this enigmatic protein in diverse biological events, including developmental processes, wound healing, immunological responses, tumorigenesis, bone resorption, and calcification. The ability of osteopontin to stimulate cell activity through multiple receptors linked to several interactive signaling pathways can account for much of the functional diversity. 产品信息 格式 Serum 控制 - Mouse kidney, COS-1 and human urine lysate
演示 Serum containing 0.05% sodium azide. 应用 应用 Detect Osteopontin using this Anti-Osteopontin Antibody validated for use in WB, IH. 主要应用 - Western Blotting
- Immunohistochemistry
生物信息 免疫原品种 Synthetic peptide corresponding to aa 75-90 of osteopontin protein. 表位 aa 75-90 宿主 Rabbit 特异性 The antibody reacts with the 60 kDa osteopontin protein. 品种反应性 MouseHuman Species Reactivity Note Reacts with human and mouse. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000582.2
基因符号 - osteopontin
- BNSP
- BSPI
- ETA-1
- MGC110940
- Nephropontin
- OPN
- SPP-1
- Uropontin
UniProt编号 - P10451
UniProt汇总 FUNCTION: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity By similarity.
SUBUNIT STRUCTURE: Ligand for integrin alpha-V/beta-3.
SUBCELLULAR LOCATION: Secreted.
PTM: Extensively phosphorylated on clustered serine residues. Ref.12 Ref.13
N- and O-glycosylated. Ref.12
DISEASE: This protein plays a principal role in urinary stone formation as the stone matrix.
SEQUENCE SIMILARITY: Belongs to the osteopontin family.
产品使用声明 质量保证 Routinely evaluated by Western Blot on mouse kidney lysate.
Western Blot Analysis:
1:500 - 1:5,000 dilution of this antibody detected osteopontin in 10 μg of mouse kidney lysate.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. 包装信息 数量 100 µL
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