merck millipore,默克密理博,AB10525,Anti-EBF-3 Antibody

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
M	WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB10525
描述	Anti-EBF-3 Antibody
Alternate Names	OE-2 Transcription factor COE3 Early B-cell factor 3 EBF-3 Olf-1/EBF-like 2 O/E-2
背景信息	The family of Early B-cell Factor (EBF) or Collier/Olf-1/EBF (COE) proteins, EBF1, EBF2, and EBF3, are ubiquitous zinc-binding transcription factors found in many species, including human, Drosophila melanogaster, and Caenorhabditis elegans. EBF proteins are capable of forming homo- and heterodimers that bind to DNA at specific sites that include the sequence 5'-ATTCCCNNGGGAATT-3'. EBF1, the prototypical member of this family, may employ epigenetic mechanisms, such as DNA methylation and chromatin remodeling, to regulate genes involved in B-cell development such as Pax5. The second member of this family EBF2 is involved in bone development, adipogenesis, and CNS development, processes which may also hinge on EBF1. EBF3 has been characterized as a tumor suppressor protein, due to its role in regulating multiple genes such as those for cyclins and CDKs which control cell growth, differentiation, and apoptosis. However, emerging evidence indicates that all EBF proteins may play a role in the development of various cancers.

产品信息
格式	Affinity Purified
控制	Mouse E16 brain tissue lysate
演示	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

应用
应用	Anti-EBF-3 Antibody is an antibody against EBF-3 for use in Western Blotting, IHC(P), Immunofluorescence, Dot Blot.
主要应用	Western Blotting
应用说明	Western Blot Analysis: A 1:500 dilution from a representative lot detected EBF-3 in COS-7 cells transfected with EBF-3. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.) Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected EBF-3 in mouse cryosections of wild type spinal cord tissue. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.) Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected EBF-3 in COS-7 cells transfected with EBF expressing plasmids. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.) Dot Blot Analysis: EBF-1, EBF-2, and EBF-3 peptides from a representative lot were probed with Anti-EBF-3 (1:100 dilution). No cross reactivity to peptides for EBF-1 & EBF-2 were observed.

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to mouse EBF-3.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	This antibody recognizes EB-3 but not EB-1 or EB-2.
品种反应性	Mouse
Species Reactivity Note	Demonstrated to react with Mouse. Predicted to react with Human and Rat based on 100% sequence homology.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_001106886
基因符号	Coe3 Ebf3
纯化方法	Affinity purified
UniProt编号	O08791

产品使用声明
质量保证	Evaluated by Western Blot in mouse E16 brain tissue lysate. Western Blot Analysis: 2 µg/mL of this antibody detected EBF-3 in 10 µg of mouse E16 brain tissue lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt.

包装信息
数量	100 µg

merck millipore,默克密理博,AB10525,Anti-EBF-3 Antibody

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