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描述 概述 The pBiEx™ vectors are designed for cloning and high-level expression of proteins in both bacteria and in transiently transfected Spodoptera-derived insect cells. In E. coli, target gene transcription is regulated by the tightly controlled T7lac promoter, and a ribosome binding site (rbs) directs translation initiation. In insect cells, transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1.
pBiEx-1 contains N-terminal His•Tag and S•Tag coding sequences, and a C-terminal HSV•Tag coding sequence. For sequencing or PCR of recombinants in pBiEx-1, refer to the table below to choose the appropriate primers.
Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.Vector 5' Primer 3' Primer alt 5' Primer pIEx-1 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer pIEx-2 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer pIEx-3 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer pIEx-4 IE1 Promoter Primer AS S•Tag 18mer Primer NA pIEx-5 IE1 Promoter Primer AS S•Tag 18mer Primer NA pBiEx-1 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
T7 Promoter PrimerpBiEx-2 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
T7 Promoter PrimerpBiEx-3 IE1 Promoter Primer AS S•Tag 18mer Primer T7 Promoter Primer 产品目录编号 71234 品牌系列 Novagen®
同义词 InsectDirect™ System- pBiEx™-1 DNA 储存和货运信息 货运代码 Shipped with Blue Ice or with Dry Ice
毒性 Standard Handling
储存 -20°C
无需冷冻 No
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