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merck millipore,默克密理博,AB10020,Anti-acetyl-Histone H2A.X (Lys5) Antibody
产品名称：Anti-acetyl-Histone H2A.X (Lys5) Antibody
产品型号：AB10020
Anti-acetyl-Histone H2A.X (Lys5) Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-Histone H2A.X (Lys5) also known as H2AXK5Ac, Histone H2AX (Ac K5) & has been validated in WB & Mplex.
merck millipore,默克密理博,AB10020,Anti-acetyl-Histone H2A.X (Lys5) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB10020 描述 Anti-acetyl-Histone H2A.X (Lys5) Antibody Alternate Names - H2AX histone
- H2AXK5Ac
- Histone H2AX (acetyl K5)
- H2A histone family, member X
背景信息 H2A.X is involved in DNA repair and the maintenance of genomic stability, although its precise role is still to be defined. H2AX is phosphorylated soon after the appearance of double-stranded breaks and has been implicated both in homologous recombination and nonhomologous end joining DNA repair pathways. H2AX is thought to have a critical function in the recruitment of DNA repair factors and DNA damage-signaling proteins. Hyperphoshorylation of H2AX may be linked to chromatin fragmentation prior to apoptosis. 产品信息 格式 Affinity Purified 控制 - Sodium butyrate untreated and treated HeLa acid extract
演示 Purified rabbit polyclonal containing PBS with 0.10% of sodium azide. 应用 应用 Anti-acetyl-Histone H2A.X (Lys5) Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-Histone H2A.X (Lys5) also known as H2AXK5Ac, Histone H2AX (Ac K5) & has been validated in WB & Mplex. 主要应用 - Western Blotting
应用说明 Luminex® Assay 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human Histone H2A.X acetylated at Lys5. 表位 Lys5 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes Histone H2A.X acetylated at Lys5. 品种反应性 Human Species Reactivity Note Demonstrated to react with human. Predicted to react with rat, mouse, zebrafish, bovine and canine based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_002096.1.
Entrez基因汇总 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. 基因符号 - H2a/x
- H2AFX
- H2A.X
- H2A/X
- H2AX
修改 - Acetylation
纯化方法 Affinity Purfied UniProt编号 - P16104
UniProt汇总 FUNCTION: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.
SIZE: 143 amino acids; 15145 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with numerous proteins required for DNA damage signaling and repair when phosphorylated on Ser-140. These include MDC1, TP53BP1, BRCA1 and the MRN complex, composed of MRE11A, RAD50, and NBN. Interaction with the MRN complex is mediated at least in part by NBN. Also interacts with DHX9/NDHII when phosphorylated on Ser-140.
SUBCELLULAR LOCATION: Nucleus.DEVELOPMENTAL STAGE: Synthesized in G1 as well as in S-phase.
DOMAIN: The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
PTM: Phosphorylated on Ser-140 (to form gamma-H2AFX) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Phosphorylation of Ser-140 in response to ionizing radiation is mediated by both ATM and PRKDC while defects in DNA replication induce Ser-140 phosphorylation subsequent to activation of ATR and PRKDC. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. In meiosis, Ser-140 phosphorylation may occur at synaptonemal complexes during leptotene as an ATM-dependent response to the formation of programmed DSBs by SPO11. Ser-140 phosphorylation may subsequently occurs at unsynapsed regions of both autosomes and the XY bivalent during zygotene, downstream of ATR and BRCA1 activation. Ser-140 phosphorylation may also be required for transcriptional repression of unsynapsed chromatin and meiotic sex chromosome inactivation (MSCI), whereby the X and Y chromosomes condense in pachytene to form the heterochromatic XY-body. During immunoglobulin class switch recombination in lymphocytes, Ser-140 phosphorylation may occur at sites of DNA-recombination subsequent to activation of the activation-induced cytidine deaminase AICDA. & Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression (By similarity).
SIMILARITY: Belongs to the histone H2A family.产品使用声明 质量保证 Evaluated by Western Blot in sodium butyrate untreated and treated HeLa acid extract.B109
Western Blot Analysis: 1:2,000 dilution of this antibody detected Histone H2A.X on 10 µg of sodium butyrate untreated and treated HeLa acid extract.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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