merck millipore,默克密理博,A-005-C,Poly-L-Lysine Solution (0.01%)

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merck millipore,默克密理博,A-005-C,Poly-L-Lysine Solution (0.01%)
产品名称：Poly-L-Lysine Solution (0.01%)
产品型号：A-005-C

merck millipore,默克密理博,A-005-C,Poly-L-Lysine Solution (0.01%)

产品介绍

merck millipore,默克密理博,A-005-C,Poly-L-Lysine Solution (0.01%)

重要规格表

主要应用
CULT, Cell Attachment

描述
产品目录编号	A-005-C
描述	Poly-L-Lysine Solution (0.01%)
Alternate Names	Lysine
背景信息	Poly-L-Lysine is a synthetic amino acid chain that is positively charged having one hydrobromide per unit of Lysine. Poly-L-Lysine is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. Certain cell types secrete proteases, which can digest Poly-L-Lysine. The molecular weight of Poly-L-Lysine for cell culture can vary significantly, with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (greater than 300,000 Da) having more binding sites per molecule. This product uses a Poly-L-Lysine of 84000 Da, yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.

产品信息
配方
演示	Product is presented in sterile water at 0.01% w/v (0.1mg/ml). Product is filtered through a 0.2 micron filter before freezing.

应用
主要应用	Cell Culture Cell Attachment
应用说明	Optimal coating concentrations must be determined by end user. Typical coating concentrations range from 10ug/ml to 100ug/ml based on cell type and application. 1. Thaw Poly-L-Lysine solution at room temperature. 2. Dilute Poly-L-Lysine solution to desired concentration in sterile water. 3. Fully coat the cell culture surface with diluted Poly-L-Lysine solution. Use 5 mL volume for 6-cm plates and 10 mL volume for 10-cm plates and T75 flasks. 4. Allow cell culture vessel to sit at room temperature overnight. 5. Aspirate the Poly-L-Lysine solution the following day and rinse vessel with sterile water followed by coating with desired ECM protein.

应用

主要应用

Cell Culture
Cell Attachment

应用说明

Optimal coating concentrations must be determined by end user. Typical coating concentrations range from 10ug/ml to 100ug/ml based on cell type and application.

1. Thaw Poly-L-Lysine solution at room temperature.
2. Dilute Poly-L-Lysine solution to desired concentration in sterile water.
3. Fully coat the cell culture surface with diluted Poly-L-Lysine solution. Use 5 mL volume for 6-cm plates and 10 mL volume for 10-cm plates and T75 flasks.
4. Allow cell culture vessel to sit at room temperature overnight.
5. Aspirate the Poly-L-Lysine solution the following day and rinse vessel with sterile water followed by coating with desired ECM protein.

生物信息
浓缩	0.01% w/v (0.1 mg/ml)

产品使用声明
质量保证	Solution is clear with no particulates present. Solution is sterile and suitable for cell culture applications.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.