merck millipore,默克密理愽
  • merck millipore,默克密理博,71432,Rosetta-gami™ 2 Competent Cell Set - Novagen

  • merck millipore,默克密理博,71432,Rosetta-gami™ 2 Competent Cell Set - Novagen

    产品名称：Rosetta-gami™ 2 Competent Cell Set - Novagen
    产品型号：71432


    merck millipore,默克密理博,71432,Rosetta-gami™ 2 Competent Cell Set - Novagen

  • 产品介绍
  • merck millipore,默克密理博,71432,Rosetta-gami™ 2 Competent Cell Set - Novagen

    描述
    概述 Rosetta-gami 2 host strains combine the advantages of Rosetta 2 and Origami 2 strains to alleviate codon bias and enhance disulfide bond formation in the cytoplasm when heterologoous proteins are expressed in E. coli. These trxB/gor mutants are compatible with kanamycin-resistant vectors, and carry the chloramphenicol-resistant pRARE2 plasmid, which supplies seven rare tRNAs.

    DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

    pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
    Genotypes:
    Rosetta-gami 2
    Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq pro] gor522::Tn10 trxB pRARE2 (CamR, StrR, TetR)

    Rosetta-gami 2(DE3)
    Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL (DE3) F′[lac+ lacIq pro] gor522::Tn10 trxB pRARE2 (CamR, StrR, TetR)

    Rosetta-gami 2(DE3)pLysS
    Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL (DE3) F′[lac+ lacIq pro] gor522::Tn10 trxB pLysSRARE2 (CamR, StrR, TetR)
    产品目录编号 71432
    品牌系列 Novagen®
    产品信息
    部件
    保证高效 2 × 10⁶ cfu/µg
    储存和货运信息
    货运代码 Dry Ice Only
    毒性 Multiple Toxicity Values, refer to MSDS
    储存 ≤ -70°C
    无需冷冻 No
    布鲁克黑文许可证 This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

    merck millipore,默克密理博,71432,Rosetta-gami™ 2 Competent Cell Set - Novagen

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