- 当前位置:首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
描述 概述 Rosetta-gami B strains combine the key features of BL21 (and its Tuner™ derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm. These strains are compatible with ampicillin- or spectinomycin-resistant vectors.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.Genotypes:
Rosetta-gami B
F– ompT hsdSB (rB– mB–) gal dcm lacY1 ahpC gor522::Tn10 trxB pRARE (CamR, KanR, TetR)
Rosetta-gami B(DE3)
F– ompT hsdSB (rB– mB–) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pRARE (CamR, KanR, TetR)
Rosetta-gami B(DE3)pLysS
F– ompT hsdSB (rB– mB–) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pLysSRARE (CamR, KanR, TetR)产品目录编号 71177 品牌系列 Novagen®
产品信息 部件 储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 ≤ -70°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
布鲁克黑文许可证 This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
上一件merck millipore产品:merck millipore,默克密理博,565740,α-Amyloid Precursor Protein Modulator - CAS 497259-23-1 - Calbiochem
下一件merck millipore产品:merck millipore,默克密理博,208743,Calpain Inhibitor XI - CAS 145731-49-3 - Calbiochem
merck millipore,默克密理博,40-094,BioTek 405™ LS Magnetic 96-well Washer
merck millipore,默克密理博,MAB8259,Anti-Influenza B Antibody, nucleoprotein, clone B2
merck millipore,默克密理博,AB9208,Anti-DARPP-32 Antibody, phosphoThr75
merck millipore,默克密理博,14-110,PP1 Protein, purified, 10 units
merck millipore,默克密理博,565740,α-Amyloid Precursor Protein Modulator - CAS 497259-23-1 - Calbiochem
merck millipore,默克密理博,208743,Calpain Inhibitor XI - CAS 145731-49-3 - Calbiochem
merck millipore,默克密理博,SF-772,IL17RB; Human, Cyanine 3, RNA Detection Probe,SmartFlare
merck millipore,默克密理博,MABF204,Anti-IL-33 Antibody, clone 12B3C4
merck millipore,默克密理博,236005,COX-1 Inhibitor, FR122047 - CAS 130717-51-0 - Calbiochem



