merck millipore,默克密理愽
  • merck millipore,默克密理博,70837,Origami™ B(DE3) Competent Cells - Novagen

  • merck millipore,默克密理博,70837,Origami™ B(DE3) Competent Cells - Novagen

    产品名称：Origami™ B(DE3) Competent Cells - Novagen
    产品型号：70837
    Origami B host strains carry the same mutations as the original Origami strain, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels using IPTG.

    merck millipore,默克密理博,70837,Origami™ B(DE3) Competent Cells - Novagen

  • 产品介绍
  • merck millipore,默克密理博,70837,Origami™ B(DE3) Competent Cells - Novagen

    描述
    概述 Origami™ B host strains carry the same mutations in trxB and gor as the original Origami strains, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels by adjusting the concentration of IPTG. Thus the Origami B strains combine the desirable characteristics of BL21, Tuner™, and Origami strains in one strain background. The mutations in trxB and gor are selectable on kanamycin and tetracycline, respectively; therefore, these strains cannot be used with plasmids that can only selected with kanamycin or tetracycline. These strains also include the lon and ompT deficiences of BL21, which increase protein stability.

    DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
    Genotype: F- ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC (DE3) gor522:: Tn10 trxB (KanR, TetR)
    产品目录编号 70837
    品牌系列 Novagen®
    产品信息
    部件
    保证高效 Guaranteed efficiency: > 2 x 10⁶ cfu/μg
    储存和货运信息
    货运代码 Dry Ice Only
    毒性 Multiple Toxicity Values, refer to MSDS
    储存 ≤ -70°C
    无需冷冻 No
    布鲁克黑文许可证 This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

    merck millipore,默克密理博,70837,Origami™ B(DE3) Competent Cells - Novagen

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