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描述 概述 
Pellet Paint NF Co-Precipitant is a nonfluorescent dye-labeled carrier compatible with fluorescent sequencing. It facilitates rapid removal of BigDye Terminators during the alcohol precipitation of cycle sequencing reaction products. Cycle sequencing reactions can be precipitated rapidly with 1 µl of carrier per reaction and centrifugation times of 10 minutes. The easily visualized carrier provides a simple confirmation that precipitation has occurred. Sequencing reaction products are efficiently pelleted and dye-labeled terminators remain in the supernatant during alcohol precipitation using the standard Applied Biosystems precipitation protocols. Resuspension of pelleted sequencing reaction products in deionized formamide can be confirmed by checking for dissolution of the carrier. Pellet Paint NF Co-Precipitant is fully compatible with the ABI PRISM® BigDye Terminator Cycle Sequencing Ready Reaction. To avoid extra sample handling, Pellet Paint NF Co-Precipitant can be added directly to the reaction mix, template DNA, crude PCR samples, or dilution buffer before the cycle sequencing reaction. Although Pellet Paint NF absorbs in the UV range, accurate spectrophotometric measurements of DNA or RNA samples are possible; the absorbance ratio (provided with each package of Pellet Paint NF) can be used as a correction factor when determining nucleic acid concentration. Pellet Paint NF Co-Precipitant has no detectable effect on the sequencing reaction or sequence accuracy. Pellet Paint NF Co-Precipitant is a useful substitute for the original Pellet Paint Co-Precipitant in applications where fluorescent detection is used.
Note: European Patent No. EP0853680 has been issued to EMD Biosciences, Inc./Novagen for a method for precipitating nucleic acid with a visible carrier. US Patent pending
产品目录编号 70748 品牌系列 Novagen®
特点及优点 - Efficient and rapid precipitation of BigDye® cycle sequencing products
- Efficient removal of dye terminators
- Direct visualization and tracking of precipitated material
- No effect on sequencing reaction
- Substitute for original Pellet Paint® Co-Precipitant for fluorescent detection applications
产品信息 部件 声明 This product is covered by U.S. Patents 7,550,447, 7,144,713, EP Patent 853,680, Canadian Patent 2,231,501 owned by EMD Chemicals Inc. or its Affiliates. 储存和货运信息 货运代码 Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
毒性 Standard Handling
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
上一件merck millipore产品：merck millipore,默克密理博,SLM-140-B,RPMI 1640 Medium (1X), liquid, With 25mM HEPES & L-Glutamine
下一件merck millipore产品：merck millipore,默克密理博,516561,Pertussis Toxin, Bordetella pertussis, Glycerol Solution - CAS 70323-44-3 - Calbiochem
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