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merck millipore,默克密理博,70-601,Universal EZ-TFA Chemiluminescent Transcription Factor Assay
产品名称：Universal EZ-TFA Chemiluminescent Transcription Factor Assay
产品型号：70-601
The Non-Radioactive Universal EZ-TFA Transcription Factor Assay provides a fast, sensitive method to detect specific transcription factor DNA binding activity in whole cell or nuclear extracts.
merck millipore,默克密理博,70-601,Universal EZ-TFA Chemiluminescent Transcription Factor Assay
- 产品介绍
重要规格表
品种反应性 主要应用 检测方法 Ca, M, H, R, Po, Eq, Rb, Gp, Ht, NhP ACT, ELISA Chemiluminescence 描述 产品目录编号 70-601 品牌系列 Upstate
商名 - Upstate
- EZ-TFA
描述 Universal EZ-TFA Chemiluminescent Transcription Factor Assay 背景信息 The UPSTATE Non-Radioactive Universal EZ-TFA Transcription Factor Assay provides a fast, sensitive method to detect specific transcription factor DNA binding activity in whole cell or nuclear extracts. This assay combines the principle of the electrophoretic mobility shift assay (EMSA) with the 96-well based enzyme-linked immunosorbent assay (ELISA), enabling manual or high-throughput sample analysis with greater sensitivity than conventional EMSA assays. After certain parameters are optimized, the entire assay takes less than 4 hours to complete with minimal hands-on time. The versatile set up allows for a flexible assay design and because the binding reaction occurs in solution, various parameters can be optimized such as probe titration, competitive oligonucleotide concentration, or treatment conditions. This kit contains the reagents and instructions necessary to develop an assay to monitor the DNA binding activity of any transcription factor of interest, or as a kit component for Upstate developed target specific Transcription Factor Assays. The Universal kit also allows for the design of native promoter capture probe sequences to interrogate specific transcription factor binding.
Test Principle:
The Non-Radioactive Universal Transcription Factor Assay kit is provided in a 96-well format. During the assay, the capture probe, a double stranded biotinylated oligonucleotide containing the consensus sequence for the specific transcription factor under study, is mixed with cellular (nuclear) extract in the transcription factor assay buffer provided directly in the streptavidin coated plate. When incubated together, the transcription factor contained in the extract binds to its consensus sequence. The biotinylated double stranded oligonucleotide bound to the transcription factor being studied is immobilized on the streptavidin-coated plate and any inactive, unbound material is washed away. The bound transcription factor is detected with a specific primary antibody. An HRP-conjugated secondary antibody is then used for detection and provides sensitive chemiluminescent detection that can be read in a microplate luminometer or by a CCD camera-coupled imaging system.产品信息 部件 - Blocking Reagent, Catalog # 90273.
- 10X Annealing Buffer, Catalog # 2007044
- Chemiluminescent Detection Reagent, Catalog #90495
- Chemiluminescent Reaction Buffer, Catalog #90496
- TFA Buffer, 5X, Catalog # 90272
- Gt X Rb, HRP Secondary Antibody, Catalog # 90276
- Gt X Ms, HRP Secondary Antibody, Catalog # 2003482
- Streptavidin Coated Chemiluminescent Plate, Catalog # 90494.
检测方法 Chemiluminescence 应用 应用 The Non-Radioactive Universal EZ-TFA Transcription Factor Assay provides a fast, sensitive method to detect specific transcription factor DNA binding activity in whole cell or nuclear extracts. 主要应用 - Activity Assay
- ELISA
生物信息 品种反应性 CanineMouseHumanRatPigHorseRabbitGuinea PigHamsterNon-human Primate 产品使用声明 质量保证 Routinely evaluated with gene specific target componet with all necessary controls. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Upon receipt, store components at the temperatures indicated on the labels.
Store at 4°C:
Transcription Factor Assay Chemiluminescent Plate, Catalog # 90494.
Blocking Reagent, Catalog # 90273.
10X Annealing Buffer, Catalog # 2007044.
Chemiluminescent Detection Reagent, Catalog #90495.
Chemiluminescent Reaction Buffer, Catalog #90496.
Store at -20°C:
Gt X Rb, HRP Secondary Antibody, Catalog # 90276.
TFA Buffer, 5X, Catalog # 90272
Gt X Ms, HRP Secondary Antibody, Catalog # 2003482.包装信息 数量 192 assays
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