- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
经验配方 CAS号 C₁₄H₁₁NO₆ 1173888-41-9 描述 概述 A cell-permeable, practically non-fluorescent (Φ0 = 0.001) coumarin cis-acrylate derivative that exhibits a 470-fold increase in fluorescence property (Φ = 0.47; Ex. 400 nm, Em. 465 nm) upon an 1:1 Michael adduct formation with thiol/sulfhydryl and acts as a fast-reacting (t1/2 = 3 msec with 50 µM probe and 50 mM 2-ME at t = 0), highly sensitive (Detection limit ≤0.5 nM GSH with 100 nM probe), and thiol-specific fluorogenic probe, displaying high reactivity toward 2-ME, GSH, and Cysteine, but not non-thiol-containing GSSG or Cystine. A more sensitive alternative to Coomassie Blue in staining of BSA on SDS-PAGE gels and has been applied successfully in fluorescent labeling cellular thiols in intact HEK293 cells as well as in detecting glutathione reductase activity in cell-free enzymatic assays. ThioGlo®1 (Cat. No. 595501), ThioGlo®3, and ThioGlo®5, in comparison, exhibit much lower fluorescence quantum yield increases upon reaction with thiol (61-, 156-, and 28-fold increase, respectively). 产品目录编号 595504 品牌系列 Calbiochem®
同义词 3-(7-Hydroxy-2-oxo-2H-chromen-3-ylcarbamoyl)acrylic acid methyl ester 参考 参考 Yi, L., et al. 2009. Angew Chem. Int. Ed. Engl. 48, 4034. 产品信息 CAS号 1173888-41-9 形式 Brown solid Hill配方 C₁₄H₁₁NO₆ 化学配方 C₁₄H₁₁NO₆ Molar Mass 289.2 结构配方图像 生物信息 纯度 ≥98% by HPLC 理化信息 溶解度 DMSO 储存和货运信息 货运代码 Ambient Temperature Only
毒性 Standard Handling
储存 -20°C
避光 Protect from light
无需冷冻 No
特殊指南 Use only fresh DMSO that for reconstitution. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. 包装信息 惰性气体下包装 Packaged under inert gas
上一件merck millipore产品：merck millipore,默克密理博,HTS045RTA,Ready-to-Assay™ OX 2 Orexin Receptor Frozen Cells
下一件merck millipore产品：merck millipore,默克密理博,FCMAB191F,Milli-Mark™ Anti-CD27-FITC Antibody, clone M-T271
merck millipore,默克密理博,14-615,MINK Protein, active, 10 µg
merck millipore,默克密理博,16-255,Anti-Histidine Tagged Antibody, clone 4D11, Alexa Fluor 555 conjugate
merck millipore,默克密理博,05-348,Anti-Tau Antibody, clone 5E2
merck millipore,默克密理博,14-581M,Ron Protein, active, 250 µg
merck millipore,默克密理博,HTS045RTA,Ready-to-Assay™ OX 2 Orexin Receptor Frozen Cells
merck millipore,默克密理博,FCMAB191F,Milli-Mark™ Anti-CD27-FITC Antibody, clone M-T271
merck millipore,默克密理博,428035,Anti-HOCl-Oxidized Low Density Lipoprotein Rabbit pAb
merck millipore,默克密理博,IL024,Interleukin-1β Protein, Recombinant rat
merck millipore,默克密理博,58346,Tetracycline, Hydrochloride - CAS 64-75-5 - Calbiochem
merck millipore,默克密理博,07-561,Anti-phospho-eNOS/NOS III (Ser617) Antibody