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描述 概述 This product has been discontinued.
The ProteoExtract® Phosphopeptide Enrichment SCIMAC Kit is a useful tool for enrichment of phosphorylated peptides from complex samples by combination of strong cation exchange chromatography (SCX) and immobilized metal ion affinity chromatography (IMAC). The identification of phosphorylation sites is routinely accomplished by mass spectrometry. Captured and eluted phosphopeptide fractions are ready for MALDI- and ESI- mass spectrometry analysis.
产品目录编号 539723 品牌系列 Calbiochem®
应用数据 
The combination of SCX and IMAC exhibits high selectivity for phosphopeptides by reducing nonspecific binding of peptides from complex mixtures. A complex peptide mixture derived from a tryptic digest of porcine liver extract was spiked with α-casein and two synthetic phosphopeptides. The samples were subsequently processed using (A) an ironoxide-based IMAC material alone or (B) the combination of SCX with zirconium-based IMAC particles as outlined in the Detailed Protocol. Mass spectrometry analysis was performed using ESI-LC/MS equipment operated in positive mode.产品信息 检测方法 Mass Spectrometry 形式 100 Isolations 格式 Phosphopeptide enrichment 试剂盒包含 10X Phosphobind Buffer, Wash Buffer 1, Wash Buffer 2, Elution Buffer, MagPrep® Phosphobind Particles, SCX Resin, SCX Elute Buffer, and a user protocol. 产品使用声明 预定用途 The ProteoExtract® Phosphopeptide Enrichment SCIMAC Kit is designed to enrich phosphorylated peptides from complex samples using a combination of strong cation exchange chromatography (SCX) and immobilized metal ion affinity chromatography (IMAC) The final phosphopeptide fraction is directly compatible with downstream analysis by MALDI- and ESI mass spectrometry. 储存和货运信息 货运代码 Blue Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
有害材料注意事项： Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存 +2°C to +8°C
存储条件 Upon arrival store the entire contents of the kit at 4°C for long-term storage or at room temperature for up to 6 months. 无需冷冻 No
补充信息 试剂盒包含 10X Phosphobind Buffer, Wash Buffer 1, Wash Buffer 2, Elution Buffer, MagPrep® Phosphobind Particles, SCX Resin, SCX Elute Buffer, and a user protocol.
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