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描述 概述 Native PKGIα purified from bovine lung. PKG is involved in regulation of smooth muscle relaxation, platelet function, and cell division. PKG is activated by binding of cGMP to regulatory subunits within the holoenzyme, but in contrast to PKA, the activation of PKG does not invlove physical dissociation of catalytic from the regulatory domains. The activated enzyme phosphorylates target proteins at serine and/or threonine residues that are found within the recognition motif, Arg-Lys-Arg-Ser*-Arg-Ala-Glu. Note: 4µg = 6000 units. 产品目录编号 539578 品牌系列 Calbiochem®
同义词 cGMP-Dependent Protein Kinase Catalytic Subunit, PKGIα Catalytic Subunit, Protein Kinase G Iα 应用数据
参考 参考 Brophy, M.C., et al. 2006. J. Vasc. Res. 39, 95.
Colbran J.L., et al. 1992. J. Biol. Chem. 267, 9589.
Corbin, J.D. and Doskeland, S.O. 1983. J Biol Chem 258, 11391.
Witt, J.J. and Roskoski, R., Jr, 1975. Anal Biochem 66, 253.产品信息 形式 Liquid 配方 In 150 mM NaCl, 25 mM β-mercaptoethanol, 10 mM K₂HPO₄, 1 mM EDTA, 12% sucrose, pH 6.8. Molar Mass 78000 生物信息 特异活性 ≥1300 units/µg enzyme 浓缩标签 Please refer to vial label for lot-specific concentration
储存和货运信息 货运代码 Dry Ice Only
毒性 Standard Handling
储存 ≤ -70°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-70°C).
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