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描述 概述 Serine protease that exhibits strong proteolytic activity on a wide variety of denatured and native proteins of high molecular weight. Because of its rapid proteolytic inactivation of endogenous nucleases, it can be used for isolation of mRNA and high molecular weight DNA. Preferentially cleaves bonds next to the carbonyl group of N-substituted hydrophobic, aliphatic, and aromatic amino acids. Inhibited by DFP, Hg2+, and PMSF. Not inactivated by metal chelators, sulfhydryl reagents, TLCK, or TPCK. 产品目录编号 539480 品牌系列 Calbiochem®
参考 参考 Watazu, Y., et al. 1993. J. Lab. Clin. Anal. 7, 81.
Lebherz, H.G., et al. 1986. Biochem. J. 233, 51.
Ebeling, W., et al. 1974. Eur. J. Biochem. 47, 91.产品信息 CAS号 39450-01-6 活性 ≥30 mAnsonU/mg dry weight EC号 3.4.21.14 形式 White lyophilized solid 配方 Lyophilized from calcium acetate Molar Mass 28500 生物信息 特异活性 ≥40 mAnsonU/mg protein 理化信息 污染物 DNase: no detectable nicking activity with pBR322, incubation for 6 h at 37°C; RNase: no ribonuclease activity with MS2 RNA detected after incubation for 6 h at 37°C pH值 optimum 7.5 - 12.0 溶解度 50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0 安全信息 R相 R: 36/37/38-42
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation.S相 S: 22-24-26-36/37
Do not breathe dust.
Avoid contact with skin.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing and gloves.储存和货运信息 货运代码 Ambient Temperature Only
毒性 Irritant
储存 +2°C to +8°C
无需冷冻 No
特殊指南 Following reconstitution, store in the refrigerator (4°C). Stock solutions prepared in 50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0 are stable for up to 1 year at 4°C.
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下一件merck millipore产品：merck millipore,默克密理博,S24-KC,Rat Serum, (1L) KC
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