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检测方法 Fluorometric 描述 概述 Useful in quantifying 20S proteasome activity in cuvettes or a ½ volume 96-well plate. 20S activity is measured by monitoring the release of free AMC from the fluorogenic peptide Suc-Leu-Leu-Val-Tyr-AMC over time. 产品目录编号 539158 品牌系列 Calbiochem®
参考 参考 DeMartino, G.N., and Slaughter, C. 1999. J. Biol. Chem. 274, 22123.
Adams, G., et al. 1998. Biochemistry 37, 12927.
Glickman, M., et al. 1998. Cell 94, 615.
Groll, M., et al. 1997. Nature 386, 463.
Coux, O., et al. 1996. Annu. Rev. Biochem. 65, 801.
Stein, R. et al. 1996. Biochemistry 35, 3899.
Seemuller, E., et al. 1995. Science 268, 579.
Shibatani, T., and Ward, W. 1995. Arch. Biochem. Biophys. 321, 160.
Hoffman, L., et al. 1992. J. Biol. Chem. 267, 22362.
Ma, C.P., et al. 1992. J. Biol. Chem. 267, 10515.产品信息 检测方法 Fluorometric 格式 Cuvette or 96-well plate 试剂盒包含 20S Proteasome in Buffer, Reaction Buffer, SDS, Substrate Solution, and a user protocol. Does not include the 7-Amino-4-methylcoumarin (AMC; Cat. No. 164545) standard. 生物信息 化验时间 30 min 安全信息 R相 R: 36/37/38-22
Irritating to eyes, respiratory system and skin.
Harmful if swallowed.S相 S: 26-36
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 Multiple storage requirements
存储条件 Upon arrival of the kit store the Enzyme Solution -80°C. The remainder of the kit should be stored at -20°C. 避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 20S Proteasome in Buffer, Reaction Buffer, SDS, Substrate Solution, and a user protocol. Does not include the 7-Amino-4-methylcoumarin (AMC; Cat. No. 164545) standard.
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