merck millipore,默克密理博,482655,Nitric Oxide Assay Kit, Fluorometric

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Merck Millipore 默克密理愽

merck millipore,默克密理博,482655,Nitric Oxide Assay Kit, Fluorometric
产品名称：Nitric Oxide Assay Kit, Fluorometric
产品型号：482655

merck millipore,默克密理博,482655,Nitric Oxide Assay Kit, Fluorometric

检测方法
Fluorometric

描述
概述	Assay kit useful for the rapid quantitative measurement of nitric oxide (NO). Displays 50-fold increased sensitivity over the colorimetric nitric oxide assay kit (Cat. No. 482650). The assay is based on the enzymatic conversion of nitrate to nitrite by nitrate reductase, followed by the addition of 2,3-diaminonapthalene (DAN), and NaOH, which converts nitrite to a fluorescent compound. Fluorescence measurements of this compound accurately determine the nitrite (NO₂^-) concentration (excitation max.: 365 nm; emission max.: 450 nm). Do not use with nitrate- or nitrite-containing tissue culture media such as RPMI.
产品目录编号	482655
品牌系列	Calbiochem®

参考
参考	Miles, A.M., et al. 1996. Methods Enzymol. 268, 105. Misko, T.P., et al. 1993. Anal. Biochem. 214, 11. Green, L.C., et al. 1982. Anal. Biochem. 126, 131.

产品信息
检测方法	Fluorometric
形式	192-288 Tests
格式	96-well plate
试剂盒包含	Assay Buffer, Nitrate Reductase, Enzyme Cofactors, Nitrate Standard, Nitrite Standard, DAN Reagent, NaOH, Microtiter Plates, Plate Covers, and a user protocol.

生物信息
化验时间	3-5 h
样品类型	Aqueous solutions

安全信息
R相	R: 34-36/37/38-45 Causes burns. Irritating to eyes, respiratory system and skin. May cause cancer.
S相	S: 26-36/37/39-45-53 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). Avoid exposure - obtain special instructions before use.

储存和货运信息
货运代码	Blue Ice Only
毒性	Multiple Toxicity Values, refer to MSDS
有害材料注意事项：	Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存	-20°C
存储条件	Upon arrival, store the entire contents of the kit at -20°C until use. For storage information on individual components following initial thawing and reconstitution, please consult the section on Pre-Assay Preparation.
无需冷冻	No

补充信息
试剂盒包含	Assay Buffer, Nitrate Reductase, Enzyme Cofactors, Nitrate Standard, Nitrite Standard, DAN Reagent, NaOH, Microtiter Plates, Plate Covers, and a user protocol.

merck millipore,默克密理博,482655,Nitric Oxide Assay Kit, Fluorometric

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