merck millipore,默克密理博,48-669MAG,MILLIPLEX MAP Human Early Apoptosis Magnetic Bead 7-Plex Kit

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merck millipore,默克密理博,48-669MAG,MILLIPLEX MAP Human Early Apoptosis Magnetic Bead 7-Plex Kit - Cell Signaling Multiplex Assay
产品名称：MILLIPLEX MAP Human Early Apoptosis Magnetic Bead 7-Plex Kit - Cell Signaling Multiplex Assay
产品型号：48-669MAG
The analytes available for this multiplex kit are: Akt (Ser473), BAD (Ser112), Bcl-2 (Ser70), Active Caspase 8 (Asp384), Active Caspase 9 (Asp315), JNK (Thr183/Tyr185), p53 (Ser46).

merck millipore,默克密理博,48-669MAG,MILLIPLEX MAP Human Early Apoptosis Magnetic Bead 7-Plex Kit - Cell Signaling Multiplex Assay

产品介绍

merck millipore,默克密理博,48-669MAG,MILLIPLEX MAP Human Early Apoptosis Magnetic Bead 7-Plex Kit - Cell Signaling Multiplex Assay

重要规格表

可用的分析物	品种反应性	主要应用	检测方法
p53 (Ser46), Akt (Ser473), BAD (Ser112), Bcl-2 (Ser70), Active Caspase 8 (Asp384), Active Caspase 9 (Asp315), JNK (Thr183/Tyr185)	H	Mplex	Luminex xMAP

描述
产品目录编号	48-669MAG
商名	MILLIPLEX
描述	MILLIPLEX MAP Human Early Apoptosis Magnetic Bead 7-Plex Kit - Cell Signaling Multiplex Assay
概述	Apoptosis is an essential physiological process that plays a crucial role in normal development and homeostatic mechanisms of multicellular organisms. At least two major apoptotic pathways have been described in mammalian cells: the intrinsic pathway via Caspase-9 activation, in which death arises from mitochondrial dysfunction, and the extrinsic pathway via Caspase-8 activation, in which death is initiated from the activation of cell surface receptors. Cleaved and activated Caspase-8 or -9 can activate Caspase-3 and -7 which are responsible for the cleavage of various proteins leading to the biochemical and morphological features characteristic of apoptosis. Bcl-2 family members play a pivotal role in the regulation of apoptosis as they reside upstream of irreversible cellular damage and focus much of their efforts at the level of mitochondria. The BH3-only protein Bad promotes cell death by interacting with and inhibiting the pro-survival Bcl-2 proteins. Survival factors suppress Bad-mediated apoptosis by inducing phosphorylation of Bad by Akt. Phosphorylated Bad then binds to 14-3-3 instead of Bcl-2 or Bcl-XL, resulting in the liberation of the anti-apoptotic proteins and the promotion of cell survival. Irreversible cellular damage can also activate apoptosis by transcriptional activation of many pro-apoptotic genes by the transcription factors AP1 (the stress activated pathway, JNK) and p53 (DNA-damage response pathway).

产品信息
检测方法	Luminex xMAP
配置	Premixed
板面类型	Cell Signaling

应用
应用	The analytes available for this multiplex kit are: Akt (Ser473), BAD (Ser112), Bcl-2 (Ser70), Active Caspase 8 (Asp384), Active Caspase 9 (Asp315), JNK (Thr183/Tyr185), p53 (Ser46).
主要应用	Multiplexing
应用说明	• An overnight (4°C) incubation is recommended for best results. • This assay requires 25 µL diluted cell lysate per well. • This kit must be run using Assay Buffer 2 (provided). • 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL)

生物信息
特异性	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
品种反应性	Human
可用的分析物	p53 (Ser46) Akt (Ser473) BAD (Ser112) Bcl-2 (Ser70) Active Caspase 8 (Asp384) Active Caspase 9 (Asp315) JNK (Thr183/Tyr185)

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.