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品种反应性 主要应用 宿主 格式 抗体类型 R, Ch, H, M WB Rb Purified Polyclonal Antibody 描述 产品目录编号 36-003 品牌系列 Upstate
商名 - Upstate
描述 Anti-phospho-MYPT1 (Thr850) Antibody 产品信息 格式 Purified 控制 - MYPT1 substrate phosphorylated by ROKa/ROCK-II
演示 Protein A Purified rabbit IgG in a solution of 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.135M NaCl, and 0.035% sodium azide as a preservative. 应用 应用 Detect phospho-MYPT1 (Thr850) using this Anti-phospho-MYPT1 (Thr850) Antibody validated for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 Peptide corresponding to amino acid residues 845-855 of chicken MYPT1; this sequence is identical to amino acids 848-858 of human MYPT1, with the phosphorylated Thr at position 853. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recombinant truncated chicken MYPT1 phosphorylated on Thr850. 同种型 IgG 品种反应性 RatChickenHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002480.1
Entrez基因汇总 Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. 基因符号 - MYPT1
- PPP1R12A
- MBS
- MGC133042
修改 - Phosphorylation
纯化方法 Protein A purfied UniProt编号 - O14974
UniProt汇总 FUNCTION: SwissProt: O14974 # Regulates myosin phosphatase activity.
SIZE: 1030 amino acids; 115281 Da
SUBUNIT: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16.
SUBCELLULAR LOCATION: Cytoplasm. Note=Along actomyosin filaments and stress fibers.
PTM: Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: O14974 ## Contains 6 ANK repeats.产品使用声明 质量保证 Routinely evaluated by immunoblot on phophorylated MYPT1 substrate 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 200 µg
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