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纯度 ≥95% by SDS-PAGE 描述 概述 Recombinant, human epidermal growth factor (54 amino acids) expressed in E. coli. Identical to human EGF except for an additional N-terminal methionine. Human EGF is 70% homologous to mouse EGF and has been shown to have similar biological activity. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Acts as a survival factor in preventing apoptosis. 产品目录编号 324831 品牌系列 Calbiochem®
同义词 Epidermal Growth Factor, Human, Recombinant, E. coli 参考 参考 Merlo, G.R., et al. 1995. J. Cell Biol. 128, 1185.
Yoshida, T., et al. 1993. Brain Res. Dev. 76, 147.
Rubin, J.S., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 415.
Carpenter, G., and Wahl, M.I. 1990. In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.) Springer-Verlag, New York, p 69.
Downward, J., et al. 1984. Nature 311, 483.
Gray, A., et al. 1983. Nature 303, 722.
Scott, J., et al. 1983. Science 221, 236.
Cohen, S. 1960. Proc. Natl. Acad. Sci. USA 46, 302.产品信息 CAS号 62229-50-9 形式 Solid 配方 Lyophilized from sterile-filtered PBS. Molar Mass 6000 生物信息 生物活性 ED₅₀ = 100-400 pg/ml as measured by its ability to stimulate the ³H-thymidine incorporation in an EGF-responsive mouse fibroblast cell line, Balb/3T3. 纯度 ≥95% by SDS-PAGE 理化信息 污染物 Endotoxin: <1.0 EU/μg EGF 溶解度 10 mM acetic acid containing ≥0.1% HSA or BSA GHS安全信息 RTECS KB4518000 储存和货运信息 货运代码 Ambient Temperature Only
毒性 Standard Handling
储存 -20°C
无需冷冻 No
特殊指南 Following reconstitution, aliquot and freeze (-20°C or -70°C) for long term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C or -70°C.
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