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Replacement Information Replacement Information 05-1426 is a recommended replacement for MAB3756 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1426 Replaces MAB3756 描述 Anti-SLP-76 Antibody, clone AS55 Alternate Names - lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
- 76 kDa tyrosine phosphoprotein 2
- Human 76 kDa tyrosine phosphoprotein SLP-76 mRNA, complete cds
- Lymphocyte cytosolic protein 2
- SH2 domain-containing leukocyte protein of 76 kDa
- SLP-76 tyrosine phosphoprotein
- SLP-76 tyrosine phosphoprotein
背景信息 Detects SLP-76 (SH2 domain containing Leukocyte Protein) of 76 kDa, a hematopoietic cell-specific adapter protein which intereacts with other signaling molecules through amino-terminal phosphotyrosine residues, a central proline-rich region and a carboxyl-terminal Src homology 2 domain (SH2). 产品信息 格式 Purified 控制 - RAW264 cell lysate
演示 Purified mouse monoclonal IgG2a 0.2 mm sterile filtered solution in phosphate buffered saline with 0.1% sodium azide. 应用 应用 Detect SLP-76 using this Anti-SLP-76 Antibody, clone AS55 validated for use in WB & IP. 主要应用 - Western Blotting
应用说明 Immunoprecipitation: A previous lot of this antibody was used in IP.
Optimal working dilutions must be determined by end user.
生物信息 免疫原品种 Monoclonal antibody from BALB/c-derived hybridoma 克隆 AS55 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes human SH2 (Src homology 2) domain-Leukocyte Protein of 76 kDa. 同种型 IgG2a 品种反应性 RatHumanMouse Species Reactivity Note Human and mouse. Expected to react with rat based on sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_005565.3
Entrez基因汇总 SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function 基因符号 - SLP76
- LCP2
- SLP-76
纯化方法 Protein G purfied UniProt编号 - Q13094
UniProt汇总 FUNCTION: SwissProt: Q13094 # Involved in T-cell antigen receptor mediated signaling.
SIZE: 533 amino acids; 60188 Da
SUBUNIT: Interacts with SLA. Interacts with CBLB (By similarity). Interacts with the adapter proteins GRB2 and FYB. Interacts with SHB. Interacts with PRAM1.
SUBCELLULAR LOCATION: Cytoplasm (Probable).
TISSUE SPECIFICITY: Highly expressed in spleen, thymus, and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblasatoma cell lines.
DOMAIN:SwissProt: Q13094 The SH2 domain mediates interaction with SHB.
PTM: Phosphorylated after T-cell receptor activation by ZAP-70.
SIMILARITY: Contains 1 SH2 domain.产品使用声明 质量保证 Routinely evaluated by Western Blot on RAW 264 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected SLP-76 on 10 μg of RAW 264 lysates.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µg
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