merck millipore,默克密理博,2520,Blot Restore Membrane Rejuvination Kit, 10x

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merck millipore,默克密理博,2520,Blot Restore Membrane Rejuvination Kit, 10x
产品名称：Blot Restore Membrane Rejuvination Kit, 10x
产品型号：2520

merck millipore,默克密理博,2520,Blot Restore Membrane Rejuvination Kit, 10x

产品介绍

merck millipore,默克密理博,2520,Blot Restore Membrane Rejuvination Kit, 10x

重要规格表

主要应用
WB

描述
产品目录编号	2520
品牌系列	Chemicon®
商名	Chemicon
描述	Blot Restore Membrane Rejuvination Kit, 10x
概述	The Blot Restore kit is an innovative tool for studies involving Western blot analysis. It is widely believed that antigen-containing membranes used for Western blot experiments should never be dried. However, this is not true. The Blot Restore kit allows multiple re-use of membranes that were developed with substrate and subsequently dried. In model experiments both PVDF and nitrocellulose membranes showed excellent performance after having been dried for at least two months. Kit contains two components. Solutioin A re-hydrates dry membranes and washes out chemiluminescent developing reagents. Solution B strips both primary and secondary (labeled) antibodies off the membrane. Treated membranes are ready for blocking and subsequent re-probing with antibodies of choice. Use of the Blot Restore Kit offers several advantages: 1) Conservation of samples that are expensive or available only in limited quantities, 2) Analysis of a given blot using several different antibodies, e.g. subtype- or isoform-specific antibodies, 3) Re-analysis of anomalous results and confirmation with the same or a different antibody, 4) Correcting errors in incubation with the wrong antibody, 5) Cost savings in reagents and time by reusing the same blot.

产品信息
部件	Blot Restore Solution B (10x) - (1 container, 50 mL). Blot Restore Solution A (10x) - (1 container, 50 mL).
检测方法	Chemiluminescence

应用
主要应用	Western Blotting
应用说明	The Blot Restore Kit is effective for hydrating dry membranes and removing antibodies from Western blots that have been developed with chemiluminescence or color substrates. The Blot Restore solution will not remove colored precipitate from blots developed using colorimetric substrates. The Blot Restore Kit should be used only for qualitative purposes until it has been established by comparative blot analysis that stripping does not quantitatively affect a given antigen. This product is for research use only; not for diagnostic or in vivo use.

应用

主要应用

Western Blotting

应用说明

The Blot Restore Kit is effective for hydrating dry membranes and removing antibodies from Western blots that have been developed with chemiluminescence or color substrates. The Blot Restore solution will not remove colored precipitate from blots developed using colorimetric substrates.

The Blot Restore Kit should be used only for qualitative purposes until it has been established by comparative blot analysis that stripping does not quantitatively affect a given antigen.

This product is for research use only; not for diagnostic or in vivo use.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.