merck millipore,默克密理博,2116,Detergent-OUT™ Med Kit

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merck millipore,默克密理博,2116,Detergent-OUT™ Med Kit
产品名称：Detergent-OUT™ Med Kit
产品型号：2116

merck millipore,默克密理博,2116,Detergent-OUT™ Med Kit

产品介绍

merck millipore,默克密理博,2116,Detergent-OUT™ Med Kit

重要规格表

Key Applications
PCU

描述
产品目录编号	2116
品牌系列	Chemicon®
商名	Chemicon
描述	Detergent-OUT™ Med Kit
概述	The Chemicon Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions. Removes detergents without significant dilution of protein solution. Simply load the protein solution on the Detergent-OUT Spin column and collect detergent free protein solution. The Med column is suitable for removing detergents from a 1.0 mL solution of 1.5% Triton-X100 detergents (or an equivalent of 15 μL Triton-X100 or 15 mg detergents), provided that both the columns and proteins solutions are prepared in water.

产品信息
部件	Detergent-OUT Columns-DTG-100X 10 Med

应用
主要应用	Protein Clean-up
应用说明	PROTOCOL: 1. Invert the column several times to re-suspend the resin material. Remove the tip of the column and let the liquid drain off. 2. Buffer Equilibration: Detergent-OUT columns are supplied in deionized water. A better result is obtained when the column and protein solution are in water or phosphate buffer. Columns may be equilibrated with other types of buffer. Note: Do not equilibrate the columns with Tris buffer and other such buffers. Apply about 1.0 mL of a desired buffer into the column. Let the buffer drain into collection tube. Repeat this process 3 times. 3. Place the column in a 15mL centrifuge tube. Centrifuge at 1000 xg for 20-30 seconds. Discard the liquid collected in the centrifuge tube. 4. Place the column back in the same centrifuge tube. Carefully apply the protein suspension to the center of the column. Wait for 5 minutes.. 5. After loading the column centrifuge at 1000 xg for 20-30 seconds. Collect the liquid comprising purified protein material free from detergent originally present in the sample.

应用

主要应用

Protein Clean-up

应用说明

PROTOCOL:

1. Invert the column several times to re-suspend the resin material. Remove the tip of the column and let the liquid drain off.

2. Buffer Equilibration: Detergent-OUT columns are supplied in deionized water. A better result is obtained when the column and protein solution are in water or phosphate buffer. Columns may be equilibrated with other types of buffer. Note: Do not equilibrate the columns with Tris buffer and other such buffers. Apply about 1.0 mL of a desired buffer into the column. Let the buffer drain into collection tube. Repeat this process 3 times.

3. Place the column in a 15mL centrifuge tube. Centrifuge at 1000 xg for 20-30 seconds. Discard the liquid collected in the centrifuge tube.

4. Place the column back in the same centrifuge tube. Carefully apply the protein suspension to the center of the column. Wait for 5 minutes..

5. After loading the column centrifuge at 1000 xg for 20-30 seconds. Collect the liquid comprising purified protein material free from detergent originally present in the sample.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Store at 2-8°C for up to 6 months from date of purchase. To avoid possible microbial contamination, dispense all solutions aseptically.

包装信息
数量	1 kit
包装	10 columns

merck millipore,默克密理博,2116,Detergent-OUT™ Med Kit

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