merck millipore,默克密理博,17-461,Phospho-EGFR (Tyr1173) STAR ELISA Kit

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,17-461,Phospho-EGFR (Tyr1173) STAR ELISA Kit
产品名称：Phospho-EGFR (Tyr1173) STAR ELISA Kit
产品型号：17-461

merck millipore,默克密理博,17-461,Phospho-EGFR (Tyr1173) STAR ELISA Kit

产品介绍

merck millipore,默克密理博,17-461,Phospho-EGFR (Tyr1173) STAR ELISA Kit

重要规格表

可用的分析物	品种反应性	主要应用	检测方法
EGFR	R, H, M	ELISA	Chromogenic

描述
产品目录编号	17-461
品牌系列	Upstate
商名	Upstate STAR
描述	Phospho-EGFR (Tyr1173) STAR ELISA Kit
Alternate Names	EGF Receptor ERBB, ERBB1, epidermal growth factor receptor
背景信息	I. TEST PRINCIPLE The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The EGFR plate is coated with a specific mouse monoclonal anti-EGFR capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are incubated in the microwells allowing EGFR antigen to be captured in the plate wells. The plate is then washed to remove any non-bound unspecific material. The wells are then incubated with a specific rabbit anti-EGFR antibody to detect the captured phospho-EGFR (Tyr1173) on the plate well. The unbound detection antibody is washed away followed by incubation with an HRP-conjugated anti-rabbit antibody. This allows for a sensitive enzymatic detection of the sample. After the addition of TMB substrate and stop solution the absorbance is measured at 450 nm using a plate reader. The entire assay takes less than 5 hours to complete with minimal hands-on time. Many of the reagents are supplied in ready-to use formulations for ease of use. The kit also includes a standard that is run as both a positive control and to develop a standard curve. II. EGFR BACKGROUND Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that plays a key role in the regulation of essential normal cellular processes and in the pathophysiology of hyperproliferative diseases such as cancer. Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase known to be essential for mediation of both proliferative and survival signals to cells. Activation of the EGFR signaling pathway has been linked with increased cell proliferation, angiogenesis, metastasis and decreased apoptosis. EGFR is found in most solid tumors and its autophosphorylation activates the intrinsic kinase activity toward heterologous substrates, as well as creating docking sites for adapter proteins for multiple adapter proteins to bind and nucleate signaling complexes that activate the Ras, PI3 Kinase, and PLC pathways. Many of the EGFR mutations are directly related to various diseases and are the target of numerous drugs. For instance, the amino acid substitution L858R is one of several heterozygous mutations that have been identified in Non-Small-Cell Lung Cancer (NSCLC) patients who have clinical responses to the EGFR inhibitor Iressa®. There is some evidence that these mutations result in elevated activity and enhanced sensitivity to Iressa®. In patients with tumors bearing Iressa®-sensitive mutations, resistant subclones containing an additional EGFR mutation, T790M, emerge in the presence of the drug. It has been shown experimentally that the T790M mutation leads to high-level functional resistance to Iressa. In patients with tumors bearing Iressa®-sensitive mutations (eg. L858R, L861Q), resistant subclones containing the T790M mutation emerge in the presence of the drug.

产品信息
部件	10.Plate Covers: Two plate covers. 1. Capture Plate pre-coated with anti-human EGFR antibody: (Part No. 17-461A) One pre-coated 96-stripwell immunoplate sealed in a foil pouch. 2. Anti-EGFR (Tyr1173) detection antibody: (Part No. 17-461B) One bottle (11 mL) of anti-EGFR (Tyr1173) detection antibody containing sodium azide, ready to use. 3. ELISA Diluent: (Part No. 17-461C) One bottle (25 mL) of ELISA Diluent containing sodium azide, ready to use. 4. 25X ELISA Wash Buffer: (Part No. 17-461D) One bottle (50 mL) of 25X ELISA Wash Buffer. 5. Anti-Rabbit IgG HRP conjugate: (Part No. 17-461E) One vial (125 µL) of 100X anti-rabbit HRP conjugate containing thimerosol. 6. HRP Diluent: (Part No. 17-461F) One bottle (25 mL) of HRP Diluent containing thimerosol. 7.TMB Solution: (Part No. 17-461G) One bottle (25 mL) of stabilized tetramethylbenzidine (TMB), ready to use. 8. Stop Solution: (Part No. 17-461H) One bottle (25 mL) of stop solution, ready to use. 9.EGFR (Tyr1173) Standard: (Part No. 17-461I) Two vials of EGFR (Tyr1173) standard, lyophilized.
检测方法	Chromogenic

产品信息

部件

10.Plate Covers: Two plate covers.
1. Capture Plate pre-coated with anti-human EGFR antibody: (Part No. 17-461A) One pre-coated 96-stripwell immunoplate sealed in a foil pouch.
2. Anti-EGFR (Tyr1173) detection antibody: (Part No. 17-461B) One bottle (11 mL) of anti-EGFR (Tyr1173) detection antibody containing sodium azide, ready to use.
3. ELISA Diluent: (Part No. 17-461C) One bottle (25 mL) of ELISA Diluent containing sodium azide, ready to use.
4. 25X ELISA Wash Buffer: (Part No. 17-461D) One bottle (50 mL) of 25X ELISA Wash Buffer.
5. Anti-Rabbit IgG HRP conjugate: (Part No. 17-461E) One vial (125 µL) of 100X anti-rabbit HRP conjugate containing thimerosol.
6. HRP Diluent: (Part No. 17-461F) One bottle (25 mL) of HRP Diluent containing thimerosol.
7.TMB Solution: (Part No. 17-461G) One bottle (25 mL) of stabilized tetramethylbenzidine (TMB), ready to use.
8. Stop Solution: (Part No. 17-461H) One bottle (25 mL) of stop solution, ready to use.
9.EGFR (Tyr1173) Standard: (Part No. 17-461I) Two vials of EGFR (Tyr1173) standard, lyophilized.

检测方法

Chromogenic

应用
主要应用	ELISA

生物信息
品种反应性	RatHumanMouse
可用的分析物	EGFR
Entrez基因编号	NM_201284.1 NM_005228.3 NM_201282.1 NM_201283.1
基因符号	mENA EGFR ERBB1 ERBB
修改	Phosphorylation
UniProt编号	P00533
UniProt汇总	FUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist. SIZE: 1210 amino acids; 134277 Da SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers. PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980]. SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain. MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.

理化信息
灵敏度	Sensitivity: 1 unit/mL. Range of Detection: 1.6 to 100 units/mL

产品使用声明
质量保证	Routinely evaluated by ELISA.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.