merck millipore,默克密理博,17-283,Rac1 Activation Assay Kit

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,17-283,Rac1 Activation Assay Kit
产品名称：Rac1 Activation Assay Kit
产品型号：17-283
Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Rac1-GTP from cell lysates & detection by a Rac1 specific monoclonal antibody.

merck millipore,默克密理博,17-283,Rac1 Activation Assay Kit

产品介绍

merck millipore,默克密理博,17-283,Rac1 Activation Assay Kit

重要规格表

品种反应性	主要应用
R, H, M	ABA

描述
产品目录编号	17-283
Replaces	SGT425
品牌系列	Upstate
商名	Upstate
描述	Rac1 Activation Assay Kit

产品信息
部件	Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325) Anti-Rac1, clone 23A8 (Cat.# 05-389) Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168) 100X GTPγS, 10mM (Cat.# 20-176) 100X GDP, 100mM (Cat.# 20-177)

应用
应用	Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Rac1-GTP from cell lysates & detection by a Rac1 specific monoclonal antibody.
主要应用	Affinity Binding Assay

生物信息
品种反应性	RatHumanMouse
Species Reactivity Note	HEK293 cells
Entrez基因编号	NM_198829.1 NM_006908.3 NM_018890.2
Entrez基因汇总	The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
基因符号	p21-Rac1 RAC1 MGC111543 ORFNames=MIG5 PAK1 MIG5 TC-25
UniProt编号	P63000
UniProt汇总	FUNCTION: SwissProt: P63000 # Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP- dependent manner, suggesting that the insertion does not completely abolish effector interaction. SIZE: 192 amino acids; 21450 Da SUBUNIT: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, CYFIP1/SRA-1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2. SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity). Melanosome. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C- terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. TISSUE SPECIFICITY: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues. DOMAIN: SwissProt: P63000 The effector region mediates interaction with DEF6. SIMILARITY: Belongs to the small GTPase superfamily. Rho family.

产品使用声明
质量保证	Routinely evaluated by precipitating Rac-GTP from 3T3 cell lysates that had been loaded with GTPγS. The precipitated Rac-GTP was detected by immunoblot analysis using anti-Rac.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

包装信息
数量	1 kit
包装	Kit capacity: 30 assays

merck millipore,默克密理博,17-283,Rac1 Activation Assay Kit

上一件merck millipore产品：merck millipore,默克密理博,539569,PP2C, α-Isoform, His•Tag, Human, Recombinant, E. coli
下一件merck millipore产品：merck millipore,默克密理博,06-332,Anti-GST Tag Antibody

whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech