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主要应用 可用的分析物 检测方法 PA PTP Colorimetric 描述 产品目录编号 17-125 品牌系列 Upstate
商名 - Upstate
描述 PTP Assay Kit 1 产品信息 部件 - 96-well microtiter plate
- Tyrosine Phosphopeptide (RRLIEDAEpYAARG] (Cat.# 12-217)
- Malachite Green Solution A (Cat.# 20-105)
- Malachite Green Additive (Cat.# 20-104)
- Phosphate Standard (Cat.# 20-103)
- pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)
- pNPP Tyr Assay Buffer (Cat.# 20-180)
检测方法 Colorimetric 应用 应用 PTP Assay Kit 1 can be used to detect PTP-1B activity by dephosphorylation of the phosphopeptide (RRLIEDAEpYAARG) using malachite green detection, or by hydrolysis of pNPP. 主要应用 - Phosphatase Assay
生物信息 可用的分析物 - PTP
Entrez基因编号 - NM_002827.2
Entrez基因汇总 The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. 基因符号 - PTP1B
- PTPN1
- PTP-1B
UniProt编号 - P18031
UniProt汇总 FUNCTION: SwissProt: P18031 # May play an important role in CKII- and p60c-src-induced signal transduction cascades (By similarity).
SIZE: 435 amino acids; 49967 Da
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.
PTM: Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
SIMILARITY: SwissProt: P18031 ## Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. & Contains 1 tyrosine-protein phosphatase domain.产品使用声明 质量保证 routinely evaluated by malachite green and pNPP hydrolysis assays to detect PTP-1B (Catalog # 14-109 or Catalog # 14-358) activity by dephosphorylation of the phosphopeptide 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
包装信息 数量 1 kit 包装 Kit capacity: 100 assays
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