merck millipore,默克密理博,17-10530,ChIPAb+ MED1 Antibody

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,17-10530,ChIPAb+ MED1 Antibody - ChIP Validated Antibody and Primer Set
产品名称：ChIPAb+ MED1 Antibody - ChIP Validated Antibody and Primer Set
产品型号：17-10530
Use ChIPAb+ MED1 ChIP Validated mouse monoclonal antibody for the detection of MED also known as Mediator of RNA polymerase II transcription subunit 1 & CRSP1/TRAP220.

merck millipore,默克密理博,17-10530,ChIPAb+ MED1 Antibody - ChIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,17-10530,ChIPAb+ MED1 Antibody - ChIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用
M, H	WB, ChIP

描述
产品目录编号	17-10530
商名	Upstate ChIPAb+
描述	ChIPAb+ MED1 Antibody - ChIP Validated Antibody and Primer Set
概述	All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ MED1 (CRSP1/TRAP220) set includes a MED1 antibody, a Normal Rabbit IgG, and positive control primers which amplify 85 bp of a human prostate-specific antigen (PSA) enhancer region. The MED1 antibody and negative control IgG are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of MED1 associated chromatin.
Alternate Names	Mediator of RNA polymerase II transcription subunit 1 Activator-recruited cofactor 205 kDa component Mediator complex subunit 1 p53 regulatory protein RB18A Peroxisome proliferator-activated receptor-binding protein Thyroid receptor-interacting protein 2 PBP PPAR-binding protein Thyroid hormone receptor-associated protein complex 220 kDa component Trap220 TR-interacting protein 2 TRIP-2 Vitamin D receptor-interacting protein complex component DRIP205 p53 regulatory protein RB18A CRSP1 TRAP220 MED1 ARC205

产品信息
格式	Affinity Purified
控制	Controls Includes normal rabbit IgG and primers specific for a human prostate-specific antigen enhancer region.
演示	Anti-MED1 (Rabbit Polyclonal), Part No. CS207349. One vial containing 50 µg affinity purified rabbit polyclonal in 72 uL buffer containing Tris-citrate/phosphate buffer (pH 7 to 8) and 0.09% sodium azide, before the addition of glycerol to 30% (0.7 mg/mL final). Store at -20°C. Normal Rabbit IgG, Part No. PP64B. One vial containing 125 μg of purified Rabbit IgG in 125 μL storage buffer containing 0.05% sodium azide. Store at -20°C. ChIP Primers, PSA. Part No. CS207340. One vial containing 75 μL of 5 μM of each primer specific for human prostate-specific antigen enhancer region (chr19:51354179-51354263, hg19 build). Store at -20°C. FOR: GCC TGG ATC TGA GAG AGA TAT CAT C REV: ACA CCT TTT TTT TTC TGG ATT GTT G

应用
应用	Use ChIPAb+ MED1 ChIP Validated mouse monoclonal antibody for the detection of MED also known as Mediator of RNA polymerase II transcription subunit 1 & CRSP1/TRAP220.
主要应用	Western Blotting Chromatin Immunoprecipitation (ChIP)
应用说明	Chromatin Immunoprecipitation: Sonicated chromatin prepared from hormone starved (72H), then androgen agonist induced (1 µM CI-4AS-1 overnight) LNCaP cells (~1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG (Part No. PP64B), or 2 µg Anti-MED1 (Part No.CS207349) and the Magna ChIP™ HiSens (Cat. # 17-10460). Successful immunoprecipitation of MED1 associated DNA fragments was verified by qPCR using ChIP Primers, PSA (Part No. CS207340) as a positive locus, and ChIP Primers, β-globin (Part No. CS200591) as a negative locus. Please refer to the Magna ChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens(Cat. # 17-10461) protocol for experimental details. Chromatin Immunoprecipitation: Sonicated chromatin prepared from hormone starved (72H), then androgen agonist induced (1 µM CI-4AS-1 overnight) LNCaP cells (1e5 and 1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG (Part No. PP64B), or 2 µg Anti-MED1 (Part No. CS207349) and the Magna ChIP™ HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of MED1 associated DNA fragments was verified by qPCR using ChIP Primers, PSA (Part No. CS207340). Please refer to the MagnaChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens (Cat. # 17-10461) protocol for experimental details. Western Blotting Analysis: 0.04 µg/mL of this antibody detected MED1 on 5, 15 and 50 µg of HeLa whole cell lysate.

生物信息
免疫原品种	Recombinant protein corresponding to human MED1 (a.a. 1525-1581).
表位	Human MED1 (a.a. 1525-1581).
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
品种反应性	MouseHuman
Species Reactivity Note	Demonstrated to react with Human and Mouse. Wide species reactivity expected based upon conservation of immunogen sequence.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_004765
基因符号	TRIP ARC2052 CRSP1 CRSP200 DRIP205 DRIP230 PBP PPARBP PPARGBP RB18A TRAP220
UniProt编号	Q15648

产品使用声明
质量保证	Chromatin Immunoprecipitation: Sonicated chromatin prepared from hormone starved (72h), then androgen agonist induced (1 µM CI-4AS-1 overnight) LNCaP cells (~1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG (Part No. PP64B), or 2 µg Anti-MED1 (Part No.CS207349) and the Magna ChIP™ HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of MED1 associated DNA fragments was verified by qPCR using ChIP Primers, PSA (Part No. CS207340). Please refer to the MagnaChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens (Cat. # 17-10461) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from hormone starved (72h), then androgen agonist induced (1 µM CI-4AS-1 overnight) LNCaP cells (~1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG (Part No. PP64B), or 2 µg Anti-MED1 (Part No.CS207349) and the Magna ChIP™ HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of MED1 associated DNA fragments was verified by qPCR using ChIP Primers, PSA (Part No. CS207340).
Please refer to the MagnaChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens (Cat. # 17-10461) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.