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merck millipore,默克密理博,17-10400,Anti-phospho-MLKL (Ser358) Antibody Set
产品名称：Anti-phospho-MLKL (Ser358) Antibody Set
产品型号：17-10400
Anti-phospho-MLKL (Ser358) Antibody Set is an antibody set against phospho-MLKL (Ser358) for use in WB.
merck millipore,默克密理博,17-10400,Anti-phospho-MLKL (Ser358) Antibody Set
- 产品介绍
描述 产品目录编号 17-10400 描述 Anti-phospho-MLKL (Ser358) Antibody Set 概述 This phospho-MLKL (Ser358) antibody set contains one vial of phospho-MLKL (Ser358) antibody and one vial of MLKL “Total” antibody.
RECOMMENDED USAGE:
It is recommended to first perform immunoprecipitation on your sample using the non-phosphorylated MLKL antibody for “total” MLKL protein isolation (see protocol section on page 2 of the Certificate of Analysis); then use the immunoprecipitated sample to detect the phosphorylated protein using the phospho-MLKL (Ser358) antibody via western blot.Alternate Names - MLKL
- Mixed lineage kinase domain-like protein
背景信息 Mixed lineage kinase domain-like protein (MLKL) is required for the execution of programmed necrosis. MLKL is a cell-permeable acrylamide compound that inhibits human, but not murine, MLKL adaptor function via covalent modification of Cys86. MLKL prevents the MLKL-RIP1-RIP3 necrosome complex from interacting with further downstream effectors. 产品信息 部件 - One vial of MLKL “Total” antibody
- One vial of phospho-MLKL (Ser358) antibody
格式 Affinity Purified 演示 100 µL of Anti-phospho-MLKL (Ser358) purified polyclonal antibody in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.
100 µg (concentration: 1 mg/mL) of Anti- MLKL purified polyclonal antibody in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.应用 应用 Anti-phospho-MLKL (Ser358) Antibody Set is an antibody set against phospho-MLKL (Ser358) for use in WB. 主要应用 - Western Blotting
应用说明 Western Blot Analysis:
Untreated HT-29 cell lysates or lysates from HT-29 cells treated with Cycloheximide,Z-VAD-FMK and TNF alpha were first immunoprecipitated using the Anti-MLKL “total” antibody (1:1000 dilution) to isolate the MLKL protein.
The isolated “total” MLKL protein sample was then probed with Anti-phospho-MLKL (Ser358) (1:1000 dilution).生物信息 免疫原品种 Linear peptide corresponding to the Protein Kinase Domain of phospho-MLKL (Ser358). 表位 Ser358 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 When used together and under the “Recommended Usage” instructions, this antibody set will detect MLKL phosphorylated at Ser358. 品种反应性 Human 抗体类型 Polyclonal Antibody 基因符号 - MLKL
修改 - Phosphorylation
纯化方法 Affinity purified UniProt编号 - Q8NB16
UniProt汇总 FUNCTION: Required for the execution of programmed necrosis.
SUBUNIT STRUCTURE: Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3. Within this complex, may play a role in the proper targeting of RIPK1/RIPK3 to its downstream effector PGAM5.
DOMAIN: The protein kinase domain is predicted to be catalytically inactive.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Contains 1 protein kinase domain.
产品使用声明 质量保证 Anti- MLKL (Total Antibody): Evaluated by Immunoprecipitation on HT-29 cell lysates.
Anti-phospho-MLKL (Ser358) Antibody: Evaluated by Dot Blot on phosphorylated and non-phosphorylated MLKL peptides.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 1 Set
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