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merck millipore,默克密理博,17-10388,Di-Ubiquitin (Lys63-linked) Recombinant Protein
产品名称：Di-Ubiquitin (Lys63-linked) Recombinant Protein
产品型号：17-10388
Native K48 linked Di-Ubiquitin which can be used as a substrate for proteases that cleave the isopeptide linkage between two ubiquitin molecules.
merck millipore,默克密理博,17-10388,Di-Ubiquitin (Lys63-linked) Recombinant Protein
- 产品介绍
重要规格表
描述 产品目录编号 17-10388 品牌系列 Chemicon®
商名 - Chemicon
描述 Di-Ubiquitin (Lys63-linked) Recombinant Protein 概述 ANTIBODY AVAILABLE!
Anti-Ubiquitin, Lys63-Specific, clone Apu3 ( catalogue # 05-1308)
This product is a native K63 linked Di-Ubiquitin which can be used as a substrate for proteases that cleave the isopeptide linkage between two ubiquitin molecules. It can also be used to investigate mechanism of binding and recognition by proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs).
This product is formed by chemical ligation.背景信息 Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation.
In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.产品信息 部件 - 50 µg of Di-Ubiquitin (Lys63-linked) reconstituted in 2.5 µL DMSO and 56 µL of 0.02 M TRIS, 0.25 M NaCl, pH 7.5 to a final concentration of 50 µM.
应用 应用 Native K48 linked Di-Ubiquitin which can be used as a substrate for proteases that cleave the isopeptide linkage between two ubiquitin molecules. 应用说明 LC-MS Analysis
Mobile phase A = 1% CH3CN, 0.1% formic acid in water (MilliQ) and B = 1% water (milliQ) and 0.1% formic acid in
CH3CN. Phenomenex Kinetex C18, (2.1×50 mm, 2.6 μM); flow rate = 0.6 mL/min, runtime = 6 min, column T = 40°C.
Gradient: 5% to 95% B over 3.5 min. (See Figure 2)生物信息 纯度 > 95% by RP-HPLC 基因符号 - Ubiquitin
- Ub
产品使用声明 质量保证 Western Blot Analysis:
0.5-2 μg/mL of anti-Ubiquitin, Lys63-Specific, clone Apu2 (Cat. # 05-1308) detected Lys63-linked Ubiquitin from 1 µg of Di-Ubiquitin (Lys63-linked) Recombinant Protein (Cat. # 17-10388). (See Figure 1)使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at -80°C. Avoid freeze/thaw cycles. 包装信息 数量 50 ug
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