merck millipore,默克密理博,17-10131,ChIPAb+™ Phospho-CREB (Ser133)

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merck millipore,默克密理博,17-10131,ChIPAb+™ Phospho-CREB (Ser133) - ChIP Validated Antibody and Primer Set
产品名称：ChIPAb+™ Phospho-CREB (Ser133) - ChIP Validated Antibody and Primer Set
产品型号：17-10131
This ChIPAb+ Phospho-CREB (Ser133) -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,17-10131,ChIPAb+™ Phospho-CREB (Ser133) - ChIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,17-10131,ChIPAb+™ Phospho-CREB (Ser133) - ChIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用
Ht, H, R, M	ChIP, EMSA, IHC, WB

描述
产品目录编号	17-10131
商名	Upstate ChIPAb+
描述	ChIPAb+™ Phospho-CREB (Ser133) - ChIP Validated Antibody and Primer Set
概述	All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Phospho-CREB (Ser133) set includes the Phospho-CREB (Ser133) antibody, a negative control normal rabbit IgG, and qPCR primers which amplify a 64 bp region of human cFos CRE. The Phospho-CREB (Ser133) and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Phospho-CREB (Ser133)-associated chromatin.
Alternate Names	transactivator protein active transcription factor CREB cAMP responsive element binding protein 1 cAMP-response element-binding protein-1
背景信息	CREB is a β-ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. CREB promotes outgrowth and differentiation as a mediator of neurotrophin pathways. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling.

产品信息
格式	Purified
控制	Includes negative control normal rabbit IgG and primers specific for human cFOS CRE.
演示	Anti-phospho-CREB (Ser133) (rabbit polyclonal), Part No. CS204400. One vial containing 100 µg of affinity purified rabbit serum in 0.1 M Tris-Glycine (pH 7.4) 0.15 M NaCl, and 0.05% sodium azide, before the addition of 30% glycerol. Store at -20°C. Normal Rabbit IgG, Part No. PP64B. One vial containing 125 µg Rabbit IgG in 125 µL storage buffer containing 0.05% sodium azide. Store at -20°C. ChIP Primers cFos CRE, Part No. CS203203. One vial containing 75 μL of 5 μM of each primer specific for the cFos CRE. Store at -20°C. FOR: GGC CCA CGA GAC CTC TGA GAC A REV: GCC TTG GCG CGT GTC CTA ATC T

应用
应用	This ChIPAb+ Phospho-CREB (Ser133) -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Chromatin Immunoprecipitation (ChIP) Electrophoretic Mobility Shift Assay Immunohistochemistry Western Blotting
应用说明	Chromatin Immunoprecipitation: Representative lot data. Sonicated chromatin prepared from 293T cells (5 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 4 µg of Normal Rabbit IgG, (Part No. P64B), or 4 µg of Anti-Phospho-CREB (Ser133) antibody (Part No. CS204400) and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of Phospho-CREB (Ser133) antibody associated DNA fragments was verified by qPCR using ChIP Primers cFos CRE (Part No. CS203203) as a positive locus, and cFos downstream 4Kb as a negative locus (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated. Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. Western Blot Analysis: Representative lot data. Untreated (lane 1) and Forskolin-treated (Lane 2) NIH/3T3 lysates were resolved by SDS-PAGE, transferred to PVDF, and probed with anti-phospho-CREB (Ser133) (1:1,000 dilution). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated CREB (Figure 3). Immunoprecipitation: 10 µL from a representative lot of this antibody was shown to immunoprecipitate phosphorylated CREB from 500 µg of cells treated with 50 mM forskolin. Immunohistochemistry: A 1:1000 dilution of a representative lot detected phosphorylated CREB in formalin-fixed paraffin-embedded normal and ischemic rat brain sections. Electrophoretic Mobility Shift: A representative lot of this antibody has been shown to gel shift by an independent laboratory.

生物信息
免疫原品种	KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB.
表位	phospho-Ser133
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes p43 phosphorylated CREB. May also recognize p30 and p38 kDa proteins. The p30 & p38 proteins may be phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology to the phosphorylated CREB immunizing peptide.
同种型	IgG
品种反应性	HamsterHumanRatMouse
Species Reactivity Note	Reacts with human, hamster, mouse and rat.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_134442
Entrez基因汇总	This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
基因符号	MGC9284 CREB1 CREB
修改	Phosphorylation
纯化方法	Purified by protein A-Sepharose followed by immunoadsorbant chromatography using an unphosphorylated CREB affinity gel column.
UniProt编号	P16220
UniProt汇总	FUNCTION:This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transciption activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity. SUBUNIT: Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP By similarity. SUBCELLULAR: Nucleus. PMT:Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: Belongs to the bZIP family. Contains 1 bZIP domain. Contains 1 KID (kinase-inducible) domain

产品使用声明
质量保证	Chromatin Immunoprecipitation: Sonicated chromatin prepared from 293T cells (5 X 10E6 cell equivalents per IP) was subjected to chromatin immunoprecipitation using either 4 μg of a Normal Rabbit IgG , or 4 μg of Anti-Phospho-CREB antibody and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of Phospho-CREB (Ser133) associated DNA fragments was verified by qPCR using ChIP Primers cFos CRE (Figure 1). Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from 293T cells (5 X 10E6 cell equivalents per IP) was subjected to chromatin immunoprecipitation using either 4 μg of a Normal Rabbit IgG , or 4 μg of Anti-Phospho-CREB antibody and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of Phospho-CREB (Ser133) associated DNA fragments was verified by qPCR using ChIP Primers cFos CRE (Figure 1).
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.