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描述 产品目录编号 14-496M 品牌系列 Upstate
商名 - Upstate
描述 PKCβII Protein, active, 250 µg 概述 N-terminal His6-tagged, recombinant amino acids 2-end of human PKCβII
PKC Lipid activator (Catalog # 20-133) is required for use.应用 应用 Active, N-terminal His6-tagged, recombinant amino acids 2-end of human PKCβII. For use in Kinase Assays. 主要应用 - Kinase Assay
生物信息 来源 expressed in Sf21 cells 品种 Human 特异活性 For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. Entrez基因编号 - NM_212535.1
- NM_002738.5
Entrez基因汇总 Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. 基因符号 - PKC-beta
- PRKCB1
- PRKCB
- PRKCB2
- PKCB
- MGC41878
- PKC-B
蛋白靶 PKCβII 纯化方法 Ni2+/NTA-agarose 靶子系列 AGC UniProt编号 - P05771
UniProt汇总 FUNCTION: SwissProt: P05771 # This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May be considered as a novel component of the NF-kappa-B signaling axis responsible for the survival and activation of B-cells after BCR cross-linking (By similarity).
COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).
SIZE: 671 amino acids; 76869 Da
SUBUNIT: Interacts with PDK1 (By similarity). Interacts in vitro with PRKCBP1.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane; Peripheral membrane protein (By similarity).
PTM: Phosphorylation on Thr-500 of isoform beta-I, within the activation loop, renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Similarly, isoform beta-II is autophosphorylated on 'Thr-640' and 'Ser-659', subsequent to phosphorylation on Thr-500. Autophosphorylated on other sites i.e. in the N-terminal and hinge regions have no effect on PKC activity (By similarity).
SIMILARITY: SwissProt: P05771 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.产品使用声明 质量保证 routinely evaluated by phosphorylation of Histone H1 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 6 months at -70°C 包装信息 数量 250 µg 包装 Also available in 10 μg size (cat#14-496) and in bulk (cat# 14-496-K).
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