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  • merck millipore,默克密理博,14-1058,PvuRts1I (5-hmC Restriction Endonuclease)

  • merck millipore,默克密理博,14-1058,PvuRts1I (5-hmC Restriction Endonuclease)

    产品名称：PvuRts1I (5-hmC Restriction Endonuclease)
    产品型号：14-1058
    PvuRts1I is an enzyme that can specifically digest DNA containing 5-hydroxymethylcytosine (5-hmC). This restriction endonucleaseis used to analyze 5-hmC patterns genomic DNA by cleaving both glucosyla More>>

    merck millipore,默克密理博,14-1058,PvuRts1I (5-hmC Restriction Endonuclease)

  • 产品介绍
  • merck millipore,默克密理博,14-1058,PvuRts1I (5-hmC Restriction Endonuclease)

    重要规格表

    主要应用
    EA
    描述
    产品目录编号 14-1058
    描述 PvuRts1I (5-hmC Restriction Endonuclease)
    概述 Specifications
    • Enzme Recognition Site: hmCN11-12/N910G
    • Unit Definition: 1 Unit will digest 1µg of hmC-containing DNA to completion in 20 minutes at room temperature in 1X reaction buffer.
    • Purity >95% purity by SDS PAGE
    • Source: Proteus vulgaris
    • Host: E. coli
    Alternate Names
    • Pvu
    • PvuRts
    背景信息 PvuRts1I is an enzyme isolated from the gram negative bacterium Proteus vulgaris that can specifically digest DNA containing 5-hmC (Szwagierczak et al., Nucleic Acids Research, 2011) while leaving cytosine and 5-mC intact. This endonuclease can be used to analyze 5-hmC patterns in mammalian genomes by cleave cutting both glucosylated and non-glucosylated 5-hmC containing DNA sequences.

    Research studies of DNA methylation and gene expression support the presence of 5-hydroxymethylcytosine (5-hmC) as a sixth base in mammalian genomes. 5-hmC is generated by Ten Eleven Translocation (TET) family of enzymes through oxidation of 5-methylcytosine (5-mC). Chemically, 5-hmC differs from 5-mC by the presence of a hydroxyl on the methyl group at position 5 of cytosine.
    产品信息
    演示 Materials Provided:
    PvuRts1I 5-hmC Endonuclease: 100U (1U/µL) in a buffer containing 20mM Tris pH 8.0, 150mM NaCl, 1mM DTT and 50% Glycerol.

    PvuRts1I 5-hmC 10X Reaction Buffer: 500µL of buffer containing 200mM Tris pH 8.0, 1.5M NaCl, 50mM MgCl2 and 10mM DTT.
    应用
    应用 PvuRts1I is an enzyme that can specifically digest DNA containing 5-hydroxymethylcytosine (5-hmC). This restriction endonucleaseis used to analyze 5-hmC patterns genomic DNA by cleaving both glucosylated & non-glucosylated 5-hmC DNA sequences.
    主要应用
    • Enzyme Assay
    生物信息
    宿主 E. coli
    纯度 Greater than 95% purity by SDS PAGE
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable at -20ºC for 1 year from date of receipt.
    包装信息
    数量 200 Units

    merck millipore,默克密理博,14-1058,PvuRts1I (5-hmC Restriction Endonuclease)

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