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merck millipore,默克密理博,05-1431,Anti-Casein Kinase 2α Antibody, 1AD9
产品名称：Anti-Casein Kinase 2α Antibody, 1AD9
产品型号：05-1431
Anti-Casein Kinase 2α Antibody, 1AD9 detects level of Casein Kinase 2α & has been published & validated for use in IC, WB, IH(P) & IP.
merck millipore,默克密理博,05-1431,Anti-Casein Kinase 2α Antibody, 1AD9
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 05-1431 Replaces 04-1129 描述 Anti-Casein Kinase 2α Antibody, 1AD9 Alternate Names - Protein kinase CK2
- CK2 catalytic subunit alpha
- CKII alpha
- Casein kinase II subunit alpha
- Casein kinase 2, alpha 1 polypeptide
- Casein kinase II alpha 1 subunit
- Casein kinase II-alpha subunit
- Casein kinase II-alpha subunit
背景信息 Unlike most serine/threonine kinases, Casein Kinases (CKs) are not activated by specific stimuli, but are essentially constitutively active. CK1 autophosphorylates, causing inactivation, and protein phosphatases reactivate the kinase. The substrates of CKs are numerous, but their function is less clear, perhaps due to the absence of obvious regulation. CK1 isoforms are thought to be involved in the response to DNA damage, while CK2 levels are elevated in many types of cancer. 产品信息 格式 Purified 控制 - HeLa cell lysate
演示 Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate, pH 7.6 with 0.25 M NaCl and 0.1% sodium azide. 应用 应用 Anti-Casein Kinase 2α Antibody, 1AD9 detects level of Casein Kinase 2α & has been published & validated for use in IC, WB, IH(P) & IP. 主要应用 - Western Blotting
- Immunocytochemistry
应用说明 Immunoprecipitation: Recommended
Immunocytochemistry: A previous lot of this antibody was used at a dilution of 5 μg/mL (acetone or methanol fix only).
Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of Casein Kinase 2 Monoclonal Antibody: S-KB-3 Cells
生物信息 免疫原品种 Purified recombinant α-subunit of casein kinase 2. 表位 Region common to both alpha and alpha' subunits 克隆 1AD9 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 The antibody binds specifically to the catalytic α-subunit of casein kinase2 (CK-2α). The antibody immunoprecipitates a protein with a molecular weight of 44 kD, CK-2α subunit. A 26 kD protein band, the β-subunit of the heterotetrameric holoenzyme α2β2, is coprecipitated with the CK-2α subunit. In western blots with extracts of kidney tissue, the antibody detects various protein bands between 40 and 44 kDa. Using 1 μg/mL of antibody, the lower detection limit for the holoenzyme is 500 ng in Western blot and 100 ng in dot blot; the detection limits for the α-subunit are 300 ng and 60 ng, respectively. 同种型 IgG1 品种反应性 RatHumanMouse Species Reactivity Note Human, mouse, and rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001895.3
Entrez基因汇总 Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. 基因符号 - CK II 3 EC 2.7.11.1
- CSNK2A1
- CK2A1
- CKII
纯化方法 Purified UniProt编号 - P68400
UniProt汇总 FUNCTION: SwissProt: P68400 # Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.
SIZE: 391 amino acids; 45144 Da
SUBUNIT: Tetramer composed of an alpha chain, an alpha' and two beta chains. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Interacts with RNPS1.
SIMILARITY: SwissProt: P68400 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. & Contains 1 protein kinase domain产品使用声明 质量保证 Routinely evaluated by Western Blot on PC12 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected Neurofilament on 10 μg of PC12 lysates.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt. 包装信息 数量 100 µg
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