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merck millipore,默克密理博,09-430,Anti-phospho-IRS1 (Ser636/Ser639) Antibody
产品名称：Anti-phospho-IRS1 (Ser636/Ser639) Antibody
产品型号：09-430
This Anti-phospho-IRS1 (Ser636/Ser639) Antibody is validated for use in WB for the detection of phospho-IRS1 (Ser636/Ser639).
merck millipore,默克密理博,09-430,Anti-phospho-IRS1 (Ser636/Ser639) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, Ca, Ch, M, Eq WB Rb Purified Polyclonal Antibody 描述 产品目录编号 09-430 描述 Anti-phospho-IRS1 (Ser636/Ser639) Antibody Alternate Names - insulin receptor substrate 1
背景信息 Insulin receptor substrate 1, also known as IRS1, is associated with various cellular processes involved in the production of insulin. IRS1 transmits insulin signals via metabolic and mitogenic pathways. IRS1 is a docking protein that binds SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer. mTOR induces the phosphorylation of IRS-1 at Ser636/639m whereas such phosphorylation is inhibited by rapamycin. 产品信息 格式 Purified 控制 - IRS1 transfected CHO +/- Insulin lysate.
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-phospho-IRS1 (Ser636/Ser639) Antibody is validated for use in WB for the detection of phospho-IRS1 (Ser636/Ser639). 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated synthetic linear peptide corresponding to amino acids at and around phosphorylated Ser636 and Ser639 of human IRS1. 表位 Phosphorylated Ser636/Ser639 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes IRS1 phosphorylated at Ser636/Ser639. 品种反应性 RatHumanCanineChickenMouseHorse Species Reactivity Note Human. Predicted to cross-react with mouse, rat, equine (horse), canine (dog) and chicken based on sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_005535.1
基因符号 - IRS-1
- HIRS-1
修改 - Phosphorylation
纯化方法 Affinity Purfied UniProt编号 - P35568
UniProt汇总 FUNCTION: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit By similarity.
SUBUNIT: structure Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. Binds to UBTF and PIK3CA in nuclear extracts By similarity. Interacts with SOCS7.
PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor By similarity.
Phosphorylation of Tyr-896 is required for GRB2-binding By similarity.
Polymorphism The Arg-971 polymorphism impairs the ability of insulin to stimulate glucose transport, glucose transporter translocation, and glycogen synthesis by affecting the PI3K/AKT1/GSK3 signaling pathway. The polymorphism at Arg-971 may contribute to the in vivo insulin resistance observed in carriers of this variant. Arg-971 could contribute to the risk for atherosclerotic cardiovascular diseases associated with non-insulin-dependent diabetes mellitus (NIDDM) by producing a cluster of insulin resistance-related metabolic abnormalities. In insulin-stimulated human endothelial cells from carriers of the Arg-971 polymorphism, genetic impairment of the IRS1/PI3K/PDPK1/AKT1 insulin signaling cascade results in impaired insulin-stimulated nitric oxide (NO) release and suggested that this may be a mechanism through which the Arg-971 polymorphism contributes to the genetic predisposition to develop endothelial dysfunction and cardiovascular disease. The Arg-971 polymorphism not only reduces phosphorylation of the substrate but allows IRS1 to act as an inhibitor of PI3K, producing global insulin resistance.
DISEASE: Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. Ref.18 Ref.19 Ref.21 Ref.24 Ref.25
SIMILARITY: Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
产品使用声明 质量保证 Evaluated by western blot on IRS1 transfected CHO +/- Insulin lysate.
Western Blot Analysis:
1:500 dilution of this antibody was used to detect IRS1 phosphorylated on Ser636/Se639 in IRS transfected CHO +/- Insulin lysate.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 200 µL
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