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merck millipore,默克密理博,07-955,Anti-Hexim 1 Antibody
产品名称：Anti-Hexim 1 Antibody
产品型号：07-955
Anti-Hexim 1 Antibody is a Rabbit Polyclonal Antibody for detection of Hexim 1 also known as Hexamethylene bis-acetamide-inducible protein 1 & has been validated in WB & IP.
merck millipore,默克密理博,07-955,Anti-Hexim 1 Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H, Chp WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-955 描述 Anti-Hexim 1 Antibody Alternate Names - menage a quatre 1
- Cardiac lineage protein 1
- Estrogen down-regulated gene 1 protein
- Hexamethylene bis-acetamide-inducible protein 1
- Menage a quatre protein 1
- hexamethylene bis-acetamide inducible 1
- hexamethylene bisacetamide-inducible protein
- hexamethylene-bis-acetamide-inducible transcript 1
- hexamthylene bis-acetamide inducible 1
背景信息 Hexim1 is a transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. Hexim1 works in cooperation with 7SK snRNA to sequester P-TEFb in a large inactive 7SK snRNP complex, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK snRNP complex is composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), Hexim1, Hexim2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Hexim1 also interacts with the N-CoR complex through NCOR1, ESR1, NR3C1, and may interact with NF-kappa-B through REL A. Hexim1 is located in the nucleus where it can bind with alpha-importin, and can also be found in the cytoplasm. Hexim1 is up-regulated by HMBA (hexamethylene bisacetamide) at the protein level and down-regulated by estrogen. 产品信息 格式 Affinity Purified 控制 - HeLa cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-Hexim 1 Antibody is a Rabbit Polyclonal Antibody for detection of Hexim 1 also known as Hexamethylene bis-acetamide-inducible protein 1 & has been validated in WB & IP. 主要应用 - Western Blotting
应用说明 Western Blot (SNAP ID) Analysis: 4 µg/ml from a previous lot detected Hexim 1 on 10 µg of HeLa cell lysate.
Immunoprecipitation Analysis: A representative lot of this antibody was used by an independent laboratory for IP.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human Hexim 1 at and around the N-terminus. 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes Hexim1 at the N-terminus. 品种反应性 HumanChimpanzee Species Reactivity Note Demonstrated to react with human. Predicted to react with chimpanzee and Rhesus monkey based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP-006451
Entrez基因汇总 Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. 基因符号 - CLP1
- EDG1
- HIS1
- HMBA-inducible
- MAQ1
- Hexim1
- CLP-1
纯化方法 Affinity Purfied UniProt编号 - O94992
UniProt汇总 FUNCTION: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity.
SUBUNIT STRUCTURE: Homo-oligomer and hetero-oligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Binds alpha-importin and is mostly nuclear.
TISSUE SPECIFICITY: Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.
INDUCTION: Up-regulated by HMBA (hexamethylene bisacetamide) (at protein level). Down-regulated by estrogen.
DOMAIN: The coiled-coil domain mediates oligomerization.
MISCELLANEOUS: Inhibits Tat activity which is required for HIV-1 transcription.
SEQUENCE SIMILARITIES: Belongs to the HEXIM family.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.1 µg/ml of this antibody detected Hexim 1 on 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,MAB3612,Anti-BubR1 Antibody, clone 8G1
下一件merck millipore产品：merck millipore,默克密理博,662045,Anti-Uncoupling Protein-1 (UCP-1) (145-159) Rabbit pAb
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