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品种反应性 主要应用 宿主 格式 抗体类型 B, H, R, M, Eq, Ch WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-626 品牌系列 Upstate
商名 - Upstate
描述 Anti-phospho-AMPKα (Thr172) Antibody Alternate Names - protein kinase, AMP-activated, alpha 1 catalytic subunit
- 5'-AMP-activated protein kinase, catalytic alpha-1 chain
- AMP -activate kinase alpha 1 subunit
- AMP-activated protein kinase, catalytic, alpha-1
- AMPK alpha 1
- AMPK alpha-1 chain
- alpha-1
- AMPK alpha 1
- AMPK alpha-1 chain
- protein kinase
- AMP-activated
背景信息 AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. The Alpha subunit has at least two isoforms (alpha 1 and alpha 2) that differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity. 产品信息 格式 Affinity Purified 控制 - HEK293 cell lysate
演示 Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide. 应用 应用 Anti-phospho-AMPKα (Thr172) Antibody is an antibody against phospho-AMPKα (Thr172) for use in WB. 主要应用 - Western Blotting
应用说明 Western Blot (SNAP ID) Analysis: 生物信息 免疫原品种 KLH-conjugated, synthetic peptide corresponding to amino acids surrounding phosphorylated Thr172 of human AMP-activated protein kinase alpha 2 (AMPKα2). The immunogenic site is conserved in AMPKα1, isoform 1 (Thr174), AMPKα1, isoform 2 (Thr189), and AMPKα2 (Thr172). Accession number NM_006251. The immunizing sequence is identical in rat. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes AMPKα. 同种型 IgG 品种反应性 BovineHumanRatMouseHorseChicken Species Reactivity Note Proven to react with human. Predicted to react with mouse, rat, chicken, horse, and cow based on sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_996790
Entrez基因汇总 The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. 基因符号 - AMPKa1
- PRKAA1
- MGC57364
- AMPK
- AMPK1
- MGC33776
修改 - Phosphorylation
纯化方法 Affinity Purfied UniProt编号 - Q13131
UniProt汇总 FUNCTION: SwissProt: Q13131 # Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
COFACTOR: Magnesium.
SIZE: 550 amino acids; 62808 Da
SUBUNIT: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1.
SIMILARITY: SwissProt: Q13131 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. & Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by Western Blot in serum starved and serum starved, Lambda dephosphorylated HEK293 cell lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected AMPKα in serum starved and serum starved, Lambda dephosphorylated HEK293 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C for 1 year from date of receipt. 包装信息 数量 100 µg
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