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品种反应性 主要应用 宿主 格式 抗体类型 M, H WB, ICC Rb Serum Polyclonal Antibody 描述 产品目录编号 07-604 品牌系列 Upstate
商名 - Upstate
描述 Anti-Rac2 Antibody Alternate Names - rho family, small GTP binding protein Rac2
- Ras-related C3 botulinum toxin substrate 3 (rho family,small GTP-binding protein Rac2)
- Small G protein
- ras-related C3 botulinum toxin substrate 2
- ras-related C3 botulinum toxin substrate 2 (rho family,small GTP binding protein Rac2)
- rho family
背景信息 The Rho family of GTPases or small G-proteins, most notably Rac, Rho, and Cdc42, are all implicated in different aspects of regulation of cytoskeletal signaling when in their GTP-bound active state. Rac, Rho and Cdc42 encompass the assembly of actin-based structures such as lamellipodia, stress fibers, and filopodia, respectively. Together they help control the organization of the actin cytoskeleton, proliferation, apoptosis, membrane transport and gene expression. Rac, Cdc42, and Rho work in concert with one another to orchestrate the migration of the cell. Rac, involved in lamellipodia formation, works with Cdc42, involved in cell polarization and filopodia formation, through their downstream targets WAVE and WASP, respectively, that signal through the Arp2/3 complex to the actin cytoskeleton. This signaling enables the coordination of cell migration at the leading edge by inducing the migrating cell to stimulate actin-mediated membrane protrusion. The downstream target of Rac, WAVE-1, functions as a scaffolding protein that directs actin reorganization by relaying signals from Rac to the Arp2/3 complex. The continued addition of actin monomers in this branched network is thought to provide the driving force for the extension of the membrane that occurs during lamellipodial protrusion. 产品信息 格式 Serum 控制 - PMN cell lysate.
演示 Rabbit polyclonal antiserum IgG in buffer containing 0.05% sodium azide. 应用 应用 Anti-Rac2 Antibody is an antibody against Rac2 for use in IC, IP & WB. 主要应用 - Western Blotting
- Immunocytochemistry
应用说明 Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate Rac2.
Affinity Precipitation Assay:
5-10 µg of PAK-1 PBD (Rac/cdc42 Assay Reagent), agarose (Catalog # 14-325) precipitated Rac-GTP in lysates of HL-60 neutrophils that were loaded in vitro with GTPS using the Rac Activation Assay Kit (Catalog # 17-283). Proteins were detected by western blot analysis using a 1:5,000 dilution of this antiserum. See also references (Benard, V., 1999; Prada-Delgado, A., 2001).生物信息 免疫原品种 Fusion protein corresponding to residues 1-192 of human Rac2. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Rac2, MW ~21 kDa. Little to no cross-reactivity observed with recombinant Rac1 protein. 同种型 IgG 品种反应性 MouseHuman Species Reactivity Note Human and mouse. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_002863
Entrez基因汇总 The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. 基因符号 - HSPC022
- RAC2
- p21-Rac2
- GX
- Gx
- EN-7
纯化方法 Unpurified UniProt编号 - P15153
UniProt汇总 FUNCTION: SwissProt: P15153 # Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Seems to be involved in the regulation of the NADPH oxidase.
SIZE: 192 amino acids; 21429 Da
SUBUNIT: Interacts with DOCK2, which may activate it.
SUBCELLULAR LOCATION: Cytoplasm. Note=Membrane-associated when activated.
TISSUE SPECIFICITY: Hematopoietic specific.
DISEASE:SwissProt: P15153 # Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome [MIM:608203].
SIMILARITY: SwissProt: P15153 ## Belongs to the small GTPase superfamily. Rho family.产品使用声明 质量保证 Evaluated by western blot on RIPA lysates from PMN (polymorphonuclear neutrophil cytosol).
Western Blot Analysis:
1:5,000-1:10,000 dilutions of this antibody detected Rac2 in RIPA lysates from PMN (polymorphonuclear neutrophil cytosol).使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. 包装信息 数量 50 µL
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