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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Purified Polyclonal Antibody 描述 产品目录编号 07-575 品牌系列 Upstate
商名 - Upstate
描述 Anti-MDM2 Antibody 概述 Anti-MDM2 产品信息 格式 Purified 演示 0.1M Tris-glycine, pH 7.4, 0.15M NaCl,0.05% sodium azide before the addition of glycerol to 30% 应用 应用 Detect MDM2 also known as Murine Double Minute 2 with Anti-MDM2 Antibody (Rabbit Polyclonal Antibody), that has been demonstrated to work in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 peptide (C-AISETEENSDELSGERQRKRHK) corresponding to amino acids 164-185 of human MDM2. 宿主 Rabbit 特异性 MDM2 同种型 IgG 品种反应性 Human 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002392 and others
Entrez基因汇总 This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. 基因符号 - HDM2
- MDM2
- hdm2
- Hdm2
- HDMX
- MGC71221
纯化方法 Protein A purfied UniProt编号 - Q00987
UniProt汇总 FUNCTION: SwissProt: Q00987 # Inhibits TP53/p53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Functions as a ubiquitin ligase E3, in the presence of E1 and E2, toward p53 and itself. Permits the nuclear export of p53 and targets it for proteasome-mediated proteolysis.
SIZE: 491 amino acids; 55233 Da
SUBUNIT: Binds p53, p73, ARF(P14), ribosomal protein L5 and specifically to RNA. Can interact also with retinoblastoma protein (RB), E1A-associated protein EP300 and the E2F1 transcription factor. Forms a ternary complex with p53 and WWOX. Interacts with USP7 and TBRG1. Isoform Mdm2-F does not interact with TP53/p53. Interacts with, and ubiquitinates HIV-1 Tat.
SUBCELLULAR LOCATION: Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Note=Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins.
TISSUE SPECIFICITY: Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.
DOMAIN: SwissProt: Q00987 Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73 and E2F1. Region II contains most of a central acidic region required for interaction with ribosomal protein L5 and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc interacts specifically with RNA whether or not zinc is present and mediates the hetero-oligomerization with MDM4. It is also essential for its ubiquitin ligase E3 activity toward p53 and itself.
PTM: Phosphorylated in response to ionizing radiation in an ATM- dependent manner.
DISEASE: SwissProt: Q00987 # Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding.
SIMILARITY: Belongs to the MDM2/MDM4 family. & Contains 1 RanBP2-type zinc finger. & Contains 1 RING-type zinc finger. & Contains 1 SWIB domain.
MISCELLANEOUS: MDM2 RING finger mutations that failed to ubiquitinate p53 in vitro did not target p53 for degradation when expressed in cells.产品使用声明 质量保证 routinely evaluated by immunoblot in RIPA lysates from HeLa cells 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 2 years at -20°C 包装信息 数量 200 µg
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