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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Purified Polyclonal Antibody 描述 产品目录编号 07-565 品牌系列 Upstate
商名 - Upstate
描述 Anti-Necdin Antibody 背景信息 Necdin is known to be a growth suppressor protein that is a member of the melanoma-associated antigen (MAGE) family and is mainly expressed in postmitotic neurons. Any expression of this protein ectopically halts growth. Necdin has been shown to play a role in modulating gene transcription. Nectin suppresses cell proliferation by interacting with hnRNP U. Necdin seems to be an important protein in the pathogenesis of Prader-Willi syndrome. Recently, Necdin has been linked to fetal and postnatal physiological myogenesis and regeneration of muscle. 产品信息 格式 Purified 演示 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30% 应用 应用 Anti-Necdin Antibody is an antibody against Necdin for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 GST fusion protein corresponding to residues 1-143 of human necdin. 宿主 Rabbit 特异性 human necdin 同种型 IgG 品种反应性 RatHumanMouse Species Reactivity Note Demonstrated to react with human, mouse, and rat. Broad species cross-reactivity expected based on sequence similarity. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002487
Entrez基因汇总 This intronless gene is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele. Studies in mouse suggest that the protein encoded by this gene may suppress growth in postmitotic neurons. 基因符号 - Necdin.
- NDN
- necdin
- HsT16328
纯化方法 Protein A purfied UniProt编号 - Q99608
UniProt汇总 FUNCTION: SwissProt: Q99608 # Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle- promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences (By similarity).
SIZE: 321 amino acids; 36086 Da
SUBUNIT: Binds to the transactivation domains of E2F1 and p53. Binds also SV40 large T antigen and adenovirus E1A. Interacts with nucleobindin 1 and 2 (By similarity).
SUBCELLULAR LOCATION: Perikaryon. Nucleus. Note=Neural perikarya, translocates to the nucleus of postmitotic neurons and interacts with the nuclear matrix.
TISSUE SPECIFICITY: Almost ubiquitous. Detected in fetal brain, lung, liver and kidney; in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Not detected in peripheral blood leukocytes. In brain, restricted to post- mitotic neurons.
DISEASE: SwissProt: Q99608 # Defects in NDN may be a cause of the Prader-Willi syndrome (PWS) which is a contiguous gene syndrome resulting from inactivity of the paternal copies of a number of genes on 15q11, through deletion or disruption of these genes or maternal uniparental disomy 15. The PWS syndrome is characterized by muscular hypotonia, mental retardation, short stature, obesity, hypogonadotropic hypogonadism, and small hands and feet.
SIMILARITY: SwissProt: Q99608 ## Contains 1 MAGE domain.产品使用声明 质量保证 Evaluated by Western Blotting in NIH3T3 cell lysate. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 2 years at -20°C 包装信息 数量 200 µg
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