merck millipore,默克密理博,07-468,Anti-HuR Antibody

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merck millipore,默克密理博,07-468,Anti-HuR Antibody
产品名称：Anti-HuR Antibody
产品型号：07-468
Anti-HuR Antibody is a Rabbit Polyclonal Antibody for detection of HuR also known as ELAV-like protein 1, Hu-antigen R & has been validated in IP, WB, ICC.

merck millipore,默克密理博,07-468,Anti-HuR Antibody

产品介绍

merck millipore,默克密理博,07-468,Anti-HuR Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	ICC, WB	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	07-468
品牌系列	Upstate
商名	Upstate
描述	Anti-HuR Antibody
Alternate Names	Hu-antigen R ELAV-like protein 1
背景信息	HuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA binding protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene expression by destabilizing their mRNA. It is known to be upregulated in certain cancers such as breast and gastric cancers. Some of its targets include cFos, IL-3, and BRCA1 mRNAs. HuR has also been shown to be involved in the regulation of VEFG-A and NFkB expression in certain cell types. It activation and accumulation are also linked to many energy signaling molcules such as that of the PI3K/Akt pathway and AMPK..

产品信息
格式	Purified
控制	3T3/A31 cell lysate
演示	See datasheet for exact formulation.

应用
应用	Anti-HuR Antibody is a Rabbit Polyclonal Antibody for detection of HuR also known as ELAV-like protein 1, Hu-antigen R & has been validated in IP, WB, ICC.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunoprecipitation Analysis: 10 µg from a representative lot detected HuR in RIPA lysate. Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected HuR in HeLa and NIH/3T3 cell lysates. Immunohistochemistry Analysis: This antibody has been reported by an independent laboratory to detect HuR in rabbit placental tissue.

生物信息
免疫原品种	KLH-conjugated, synthetic peptide corresponding to amino acids 1-13 of human HuR.
宿主	Rabbit
特异性	HuR
品种反应性	MouseHuman
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_001410
Entrez基因汇总	The protein encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It destabilizes mRNAs and thereby regulates gene expression.
基因符号	Hua ELAVL1 MelG HUR ELAV1 HuR
纯化方法	See datasheet for specific purification method.
UniProt编号	Q15717
UniProt汇总	FUNCTION: SwissProt: Q15717 # Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. SIZE: 326 amino acids; 36092 Da SUBUNIT: Interacts with ANP32A. TISSUE SPECIFICITY: Ubiquitous. PTM: Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge. SIMILARITY: SwissProt: Q15717 ## Belongs to the RRM elav family. & Contains 3 RRM (RNA recognition motif) domains.

产品使用声明
质量保证	Western Blot Analysis: Evaluated by Western Blot in 3T3/A31 cell lysate using 1 µg/mL of antibody and 10 µg of lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.