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merck millipore,默克密理博,07-414,Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24)
产品名称:Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24)
产品型号:07-414
Detect dimethyl-Arginine with Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24) (Rabbit Polyclonal Antibody), that has been shown to work in WB.
merck millipore,默克密理博,07-414,Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24)
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB Rb Serum Polyclonal Antibody 描述 产品目录编号 07-414 品牌系列 Upstate
商名 - Upstate
描述 Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24) 背景信息 Arginine methylation is a post-translational modification that results in the formation of asymmetrical and symmetrical dimethylated arginines (a- and sDMA). 产品信息 格式 Serum 控制 - Jurkat cell lysate was probed with Anti-dimethyl-Arginine, asymmetric. Several proteins containing asymmetrically dimethylated arginines were detected, including p110, p75 and Sam68.
Included Positive Antigen Control:
Catalog # 12-303, Jurkat lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
演示 Rabbit antiserum diluted in storage buffer (PBS, 0.035% sodium azide and 30% glycerol). Store at -20°C. 应用 应用 Detect dimethyl-Arginine with Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24) (Rabbit Polyclonal Antibody), that has been shown to work in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated, synthetic peptide containing asymmetric dimethyl-arginine-glycine repeats (KGR*GR*GR*GR*GPPPPPR*GR*GR*GR*G in which R* denotes asymmetric dimethylation of arginine). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes proteins that contain arginines that are asymmetrically dimethylated including p300, p110, p75 and Sam68. Specificity for asymmetrically dimethylated arginine was determined by ELISA with an unmethylated ‘backbone’ peptide or an identical peptide containing symmetrically dimethylated arginines. 同种型 IgG 品种反应性 MouseHuman Species Reactivity Note Human and mouse. Reactivity with other species has not been confirmed. 抗体类型 Polyclonal Antibody 修改 - Methylation
纯化方法 Unpurified 产品使用声明 质量保证 Routinely evaluated by Western Blot HeLa nuclear extract and p110, p75, and Sam68 in RIPA lysates from Jukat cells.
Western Blot Analysis:
A 1:500-1:1000 dilution of this lot detected p110, p75, and Sam68 in RIPA lysates from Jurkat cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.包装信息 数量 200 µL
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