merck millipore,默克密理博,07-371,Anti-Histone H2B Antibody

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merck millipore,默克密理博,07-371,Anti-Histone H2B Antibody
产品名称：Anti-Histone H2B Antibody
产品型号：07-371
Anti-Histone H2B Antibody is a Rabbit Polyclonal Antibody for detection of Histone H2B also known as Histone H2B & has been validated in ChIP & WB.

merck millipore,默克密理博,07-371,Anti-Histone H2B Antibody

产品介绍

merck millipore,默克密理博,07-371,Anti-Histone H2B Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Yeast (S. cerevisiae), Ch, Xn, H	ChIP, WB	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	07-371
Replaces	MABE15
品牌系列	Upstate
商名	Upstate
描述	Anti-Histone H2B Antibody
Alternate Names	histone cluster 2, H2be H2B Histone H2B H2B histone family, member Q histone 2, H2be histone 2 H2be
背景信息	Histone H2B is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the 'beads on a string' structure.

产品信息
格式	Purified
控制	Acid extract from either sodium butyrate or colcemid treated HeLa cells.
演示	Purified rabbit polyclonal in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide, and 30% glycerol.

应用
应用	Anti-Histone H2B Antibody is a Rabbit Polyclonal Antibody for detection of Histone H2B also known as Histone H2B & has been validated in ChIP & WB.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting
应用说明	Chromatin Immunoprecipitation (ChIP): A previous lot of this antibody was used in ChIP.

生物信息
免疫原品种	KLH-conjugated, synthetic peptide corresponding to amino acids 118-126 (CG-AVTKYTSSK) of human Histone H2B, with an N-terminal CG added for conjugation purposes.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes and is specific for Histone H2B, MW ~15 kDa. An additional band at ~23 kDa is detected in some samples, which likely corresponds to ubiquityl-Histone H2B.
同种型	IgG
品种反应性	Yeast (S. cerevisiae)ChickenXenopusHuman
Species Reactivity Note	Human, chicken, yeast and Xenopus. Others not tested. Broad species cross-reactivity expected based on sequence homology.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_778225
Entrez基因汇总	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element.
基因符号	OTTHUMP00000013920 GL105 H2B H2B-GL105 H2B.1 H2B.q H2B/q H2BFQ MGC119802 MGC119804 MGC129733 MGC129734
纯化方法	Protein A chromatography
UniProt编号	Q8N257
UniProt汇总	FUNCTION: SwissProt: Q8N257 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SIZE: 126 amino acids; 13908 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. PTM: Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. & Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class- switch recombination. SIMILARITY: SwissProt: Q8N257 ## Belongs to the histone H2B family.

产品使用声明
质量保证	Evaluated by western blot on acid extracts from either sodium butyrate or colcemid treated HeLa cells. Western Blot Analysis: A 1:1,000-1:10,000 dilution of this antibody detected Histone H2B in acid extracts from either sodium butyrate or colcemid treated HeLa cells (Catalog # 13-113 and 13-114 respectively), chicken core histone (Catalog # 13-107) and recombinant human Histone H2B (Catalog # 14-491). Recombinant Xenopus Histone H2B (Catalog # 14-410) was used with a previous lot.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Evaluated by western blot on acid extracts from either sodium butyrate or colcemid treated HeLa cells.

Western Blot Analysis:
A 1:1,000-1:10,000 dilution of this antibody detected Histone H2B in acid extracts from either sodium butyrate or colcemid treated HeLa cells (Catalog # 13-113 and 13-114 respectively), chicken core histone (Catalog # 13-107) and recombinant human Histone H2B (Catalog # 14-491). Recombinant Xenopus Histone H2B (Catalog # 14-410) was used with a previous lot.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.