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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-2270 描述 Anti-WNK4 Antibody Alternate Names - Protein kinase with no lysine 4
- Serine/threonine-protein kinase WNK4
- Protein kinase lysine-deficient 4
背景信息 WNK4 is a serine-threonine protein kinase that is commonly expressed in the distal nephron. Mutations in WNK1 and WNK4 cause pseudohypoaldosteronism type II, which causes hypertension, hyperkalemia, hyperchloremia and metabolic acidosis. WNK4 has been shown to decrease Na-Cl cotransporter activity and the potassium channel by removing it from the cell surface. It is thought that activating WNK4 inhibits the activity of WNK1. WNK4 has been shown to play a role in regulating blood pressure in addition to potassium homeostasis. 产品信息 格式 Affinity Purified 控制 - Mouse kidney cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This WNK4 antibody is validated for use in WB & IHC for the detection of the WNK4 protein. 主要应用 - Western Blotting
应用说明 Immunohistochemistry Analysis: 1:1,000 dilution from a previous lot detected WNK4 in normal human kidney tissue. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human WNK4 at the C-terminus. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes WNK4 at the C-terminus. 品种反应性 RatHumanMouse Species Reactivity Note Demonstrated to react with human and mouse. Predicted to react with rat based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_115763
Entrez基因汇总 This gene encodes a member of the WNK family of serine-threonine protein kinases. The kinase is part of the tight junction complex in kidney cells, and regulates the balance between NaCl reabsorption and K(+) secretion. The kinase regulates the activities of several types of ion channels, cotransporters, and exchangers involved in electrolyte flux in epithelial cells. Mutations in this gene result in pseudohypoaldosteronism type IIB. 基因符号 - PRKWNK4
- WNK4
纯化方法 Affinity purified UniProt编号 - Q96J92
UniProt汇总 FUNCTION: Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K+ channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K+ secretion to maintain integrated homeostasis (By similarity).
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium (By similarity).
ENZYME REGULATION: Activation requires autophosphorylation of Ser-335. Phosphorylation of Ser-331 also promotes increased activity (By similarity).
SUBUNIT STRUCTURE: Interacts with the C-terminal region of KCNJ1 (By similarity).
SUBCELLULAR LOCATION: Cell junction › tight junction By similarity. Note: Present exclusively in intercellular junctions in the distal convoluted tubule and in both the cytoplasm and intercellular junctions in the cortical collecting duct. WNK4 is part of the tight junction complex (By similarity).
TISSUE SPECIFICITY: Expressed in kidney, colon and skin.
INVOLVEMENT IN DISEASE: Defects in WNK4 are a cause of pseudohypoaldosteronism type II (PHAII) [MIM:145260]. PHAII is an autosomal dominant disease characterized by severe hypertension, hyperkalemia, and sensitivity to thiazide diuretics which may result from a chloride shunt in the renal distal nephron.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
Contains 1 protein kinase domain.
CAUTION: Cys-203 is present instead of the conserved Lys which is expected to be an active site residue. Lys-186 appears to fulfill the required catalytic function. UniProtKB Q9JIH7
SEQUENCE CAUTION: The sequence BAC04669.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAC48387.1 differs from that shown. Reason: Frameshift at position 4.产品使用声明 质量保证 Evaluated by Western Blot in mouse kidney cell lysate.
Western Blot Analysis: 0.05 µg/mL of this antibody detected WNK4 on 10 µg of mouse kidney cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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