merck millipore,默克密理博,07-219,Anti-Histone Macro H2A.1 Antibody

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merck millipore,默克密理博,07-219,Anti-Histone Macro H2A.1 Antibody
产品名称：Anti-Histone Macro H2A.1 Antibody
产品型号：07-219
Use Anti-Histone Macro H2A.1 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, IHC to detect Histone Macro H2A.1 also known as H2A histone family member Y, histone macroH2A1.2.

merck millipore,默克密理博,07-219,Anti-Histone Macro H2A.1 Antibody

产品介绍

merck millipore,默克密理博,07-219,Anti-Histone Macro H2A.1 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	IHC, WB, ICC	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	07-219
描述	Anti-Histone Macro H2A.1 Antibody
Alternate Names	core histone macro-H2A.1 H2A histone family, member Y histone macroH2A1.2 macroH2A1.2 Histone H2A.y Histone macroH2A histone macroH2A1.1 Medulloblastoma antigen MU-MB-50.205
背景信息	Histone MacroH2A (mH2A) is a variant histone also known as H2AFY. mH2A is enriched in chromatin of the female inactive X chromosome and in highly methylated CpG loci. This histone variant is known to repress RNA polymerase II-driven transcription at the level of transcriptional initiation. In contrast to most histone variants containing only subtle changes, mH2A contains a large non-histone domain (the macro domain) on its C-terminus, resulting in a histone approximately three times the size of conventional H2A. There are 3 isoforms of mH2A, two of which (mH2A1.1, mH2A1.2 ) are formed by alternative splicing and another (mH2A2) from a separate gene. There are two phosphorylation sites within the basic hinge region of mH2A: Thr128 and Ser137. Phospho-Ser137 is enriched in mitosis, implicating mH2A in the regulation of posttranslational modifications throughout the cell cycle.

产品信息
格式	Affinity Purified
控制	HeLa acid extract
演示	Rabbit polyclonal antiserum IgG in buffer containing 0.05% sodium azide and 30% glycerol.

应用
应用	Use Anti-Histone Macro H2A.1 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, IHC to detect Histone Macro H2A.1 also known as H2A histone family member Y, histone macroH2A1.2.
主要应用	Immunohistochemistry Western Blotting Immunocytochemistry
应用说明	Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone macroH2A.1 in NIH/3T3, A431, and HeLa cells. Immunohistochemistry Analysis: A 1:50-1:200 dilution of a representative lot of this antibody was reported by an independent laboratory to stain hepatocyte nuclei from female mouse liver.

生物信息
免疫原品种	GST fusion-protein corresponding to the nonhistone region of rat Histone macroH2A1 (residues 160-370). .
表位	Macro domain
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	This antibody recognizes Histone MacroH2A.1 at the macro domain.
品种反应性	MouseHuman
Species Reactivity Note	Demonstrated to react with human and mouse.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_001152985.1
基因符号	MACROH2A H2A.y H2AF12M H2AFJ H2A/y MACROH2A1.1 mH2A
纯化方法	Unpurified
UniProt编号	Q9QZQ8
UniProt汇总	FUNCTION: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation. SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with HDAC1 and HDAC2 (By similarity). SUBCELLULAR LOCATION: Nucleus. Chromosome. Note: Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin. In quiescent lymphocytes, associated with centromeric constitutive heterochromatin. TISSUE SPECIFICTY: Ubiquitously expressed, with high levels in testis. Present in liver, kidney and adrenal gland (at protein level). In the liver, present in hepatocytes and at a lesser extent in cells of the bile ducts. In the kidney, present in proximal and distal convoluted tubules and in straight proximal tubules. In the adrenal gland, present in inner cells of the cortex and medulla. PTM: Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin (By similarity). SEQUENCE SIMILARITIES: Contains 1 histone H2A domain. Contains 1 Macro domain.

产品使用声明
质量保证	Evaluated by Western Blot in HeLa acid extract. A 1:500-1:2000 dilution of this antibody detected Histone macroH2A1 in acid extracts of HeLa cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt. Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.