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merck millipore,默克密理博,07-2072,Anti-DMAP1 Antibody, CT
产品名称：Anti-DMAP1 Antibody, CT
产品型号：07-2072
Anti-DMAP1, C-terminus Antibody is a rabbit polyclonal antibody for detection of DMAP1 also known as DNA methyltransferase 1 associated protein 1, DNMT1 associated protein 1, DNMT1-associated protein More>>
merck millipore,默克密理博,07-2072,Anti-DMAP1 Antibody, CT
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H ICC, WB, ELISA Rb Purified Polyclonal Antibody 描述 产品目录编号 07-2072 描述 Anti-DMAP1 Antibody, CT Alternate Names - DNMT1-associated protein 1
- DNA methyltransferase 1 associated protein 1
- DNMT1 associated protein 1
背景信息 DNA methylation can help to regulate transcriptional silencing via repressive transcription complexes, which include methyl-CpG-binding domain proteins and histone deacetylases (HDACs). DNMT1, the core enzyme for mammalian DNA methylation, can also establish a repressive transcription complex consisting of DNMT1, HDAC2, and a third protein, termed DMAP1 for 'DNMT1-associated protein.' The 467-amino acid DMAP1 protein shares approximately 98% amino acid sequence homology with the mouse protein. DMAP1 interacts directly with the N-terminal region of DNMT1, and DMAP1 can repress transcription independently of histone deacetylase activity. DNMT1, HDAC2, and DMAP1 form a complex in vivo, and DMAP1 can interact directly with the transcriptional corepressor TSG101. The DNMT1-DMAP1 exists throughout the S phase; HDAC2 joins DNMT1 and DMAP1 only during late S phase. This provides a regulated means to deacetylate heterochromatin following replication. 产品信息 格式 Purified 控制 - HL60 cell lysate
演示 Purified polyclonal antibody in buffer containing PBS with 0.09% (W/V) sodium azide. 应用 应用 Anti-DMAP1, C-terminus Antibody is a rabbit polyclonal antibody for detection of DMAP1 also known as DNA methyltransferase 1 associated protein 1, DNMT1 associated protein 1, DNMT1-associated protein 1 & has been validated in WB,IHC(P), ELISA, ICC. 主要应用 - Immunocytochemistry
- Western Blotting
- ELISA
应用说明 ELISA: A 1:1,000 dilution of a previous lot was used in ELISA.
Immunohistochemistry (Paraffin): A 1:50-100 dilution of a previous lot was used in immunohistochemistry.
Immunocytochemistry: A 1:500 dilution of a previous lot was used.
生物信息 免疫原品种 KLH-conjugated synthetic peptide corresponding to the C-terminal region of DMAP1. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes the C-terminal region of DMAP1. 品种反应性 MouseHuman Species Reactivity Note Human. Predicted to cross-react with mouse based on sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_061973
Entrez基因汇总 This gene encodes a subunit of several, distinct complexes involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally
inactive heterochromatin following replication. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. Alternatively spliced transcript variants encoding the same protein have been described. [provided by
RefSeq].
基因符号 - SWC4
- DKFZp686L09142
- DNMAP1
- DNMTAP1
- EAF2
- FLJ11543
- KIAA1425
- OTTHUMP00000008845
- OTTHUMP00000008846
- OTTHUMP00000008847
纯化方法 Protein G purfied UniProt编号 - Q9NPF5
UniProt汇总 FUNCTION: Involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.
SUBUNIT STRUCTURE: Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit HTATIP/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41 and VPS72/YL1. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, TCFL1/YL1 and YEATS4/GAS41. DMAP1 slso forms a complex with DNMT1 and HDAC2. Throughout S phase it interacts directly with the N-terminus of DNMT1, which serves to recruit DMAP1 to replication foci. DMAP1 interacts with ING1, a component of the MSIN3A transcription repressor complex, although this interaction is not required for recruitment of ING1 to heterochromatin. Interacts directly with the transcriptional corepressor TSG101. Interacts with the pro-apoptotic protein DAXX.
SUBCELLULAR LOCATION: Nucleus. Note= Targeted to replication foci throughout S phase by DNMT1.
SEQUENCE SIMILARITIES: Contains 1 SANT domain.
产品使用声明 质量保证 Evaluated by western blot on HL60 cell lysate.
Western Blot Analysis: 1:100-1:500 dilution of this antibody detected DMAP1 in HL60 cell lysate.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at 2-8°C from date of reciept. For long term storage, store at -20°C.
Handling Recommendations: Prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
包装信息 数量 100 µg
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