merck millipore,默克密理愽
  • merck millipore,默克密理博,05-257,Anti-p300 CT Antibody, clone RW128

  • merck millipore,默克密理博,05-257,Anti-p300 CT Antibody, clone RW128

    产品名称：Anti-p300 CT Antibody, clone RW128
    产品型号：05-257
    Use Anti-p300 CT Antibody, clone RW128 (Mouse Monoclonal Antibody) validated in ChIP, IP, WB to detect p300 CT also known as KAT3B.

    merck millipore,默克密理博,05-257,Anti-p300 CT Antibody, clone RW128

  • 产品介绍
  • merck millipore,默克密理博,05-257,Anti-p300 CT Antibody, clone RW128

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, H, MChIP, WBMPurifiedMonoclonal Antibody
    描述
    产品目录编号 05-257
    品牌系列 Upstate
    商名
    • Upstate
    描述 Anti-p300 CT Antibody, clone RW128
    Alternate Names
    • KAT3B
    背景信息 p300 (also EP300 or E1A binding protein p300) and CBP (also CREBBP or CREB binding protein) are two closely related transcriptional co-activating proteins (or coactivators). p300 and CBP interact with numerous transcription factors and act to increase the expression of their target genes.
    p300 and CBP have similar structures. Both contain five protein interaction domains, the nuclear receptor interaction domain (RID), the CREB and MYB interaction domain (KIX), the cysteine/histidine regions (CH1 and CH3) and the interferon response binding domain (IBiD). In addition p300 and CBP each contain a protein or histone acetyltransferase (PAT/HAT) domain and a bromodomain that binds acetylated lysines and a PHD finger motif with unknown function.
    p300 and CBP are thought to increase gene expression in three ways:
    (1) by relaxing the chromatin structure at the gene promoter through their intrinsic histone acetyltransferase (HAT) activity
    (2) by recruiting the basal transcriptional machinery including RNA polymerase II to the promoter
    (3) by acting as adaptor molecules.
    产品信息
    格式 Purified
    控制
    • Positive Control Included: HeLa Nuclear Extract (12-309)
    演示 Protein A Purified immunoglobulin in Protein A Purified immunoglobulin in PBS, pH 7.4, 0.05% sodium azide and 30% glycerol
    应用
    应用 Use Anti-p300 CT Antibody, clone RW128 (Mouse Monoclonal Antibody) validated in ChIP, IP, WB to detect p300 CT also known as KAT3B.
    主要应用
    • Chromatin Immunoprecipitation (ChIP)
    • Western Blotting
    生物信息
    免疫原品种 GST-fusion to the C-Terminus of human p300
    表位 C-terminus
    克隆 RW128
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Recognizes p300 and co-immunoprecipitates E1A.
    同种型 IgG1
    品种反应性 RatHumanMouse
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_001429
    Entrez基因汇总 EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CBP (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CBP can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.
    基因符号
    • OTTHUMP00000028668
    • EP300
    • p300
    • P300
    纯化方法 Protein G purfied
    UniProt编号
    • Q09472
    UniProt汇总 FUNCTION: SwissProt: Q09472 # Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
    SIZE: 2414 amino acids; 264144 Da
    SUBUNIT: Interacts with phosphorylated CREB1 (By similarity). Interacts with DTX1, EID1, ELF3, FEN1, LEF1, NCOA1, NCOA6, NR3C1, PCAF, PELP1, SPIB, SRY, TCF7L2, TP53, SRCAP and TRERF1. The TAZ- type 1 domain interacts with HIF1A. Probably part of a complex with HIF1A and CREBBP. Part of a complex containing CARM1 and NCOA2/GRIP1. Interacts with ING4 and this interaction may be indirect. Interacts with the C-terminal region of CITED4. Interacts with HTLV-1 Tax and p30II. Interacts with and acetylates HIV-1 Tat.
    SUBCELLULAR LOCATION: Nucleus.
    PTM: Phosphorylated. & Methylated at Arg-580 and Arg-604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates apoptotic response. Also methylated at Arg-2142 by CARM1, which impairs interaction with NCOA2/GRIP1. & Citrullinated at Arg-2142 by PADI4, which impairs methylation by CARM1 and promotes interaction with NCOA2/GRIP1.
    DISEASE: SwissProt: Q09472 # Defects in EP300 may play a role in epithelial cancer. & Chromosomal aberrations involving EP300 may be a cause of acute myeloid leukemias. Translocation t(8;22)(p11;q13) with MYST3. & Defects in EP300 are a cause of Rubinstein-Taybi syndrome (RSTS) [MIM:180849]. RSTS is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.
    SIMILARITY: SwissProt: Q09472 ## Contains 1 bromo domain. & Contains 1 KIX domain. & Contains 2 TAZ-type zinc fingers. & Contains 1 ZZ-type zinc finger.
    产品使用声明
    质量保证 routinely evaluated by immunoblot on HeLa nuclear extract
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
    包装信息
    数量 200 µg

    merck millipore,默克密理博,05-257,Anti-p300 CT Antibody, clone RW128

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