merck millipore,默克密理博,05-661,Anti-β-Tubulin Antibody, clone AA2

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merck millipore,默克密理博,05-661,Anti-β-Tubulin Antibody, clone AA2
产品名称：Anti-β-Tubulin Antibody, clone AA2
产品型号：05-661
This Anti-β-Tubulin Antibody, clone AA2 is validated for use in WB for the detection of β-Tubulin.

merck millipore,默克密理博,05-661,Anti-β-Tubulin Antibody, clone AA2

产品介绍

merck millipore,默克密理博,05-661,Anti-β-Tubulin Antibody, clone AA2

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
B, H, R, M	WB	M	Purified	Monoclonal Antibody

描述
产品目录编号	05-661
品牌系列	Upstate
商名	Upstate
描述	Anti-β-Tubulin Antibody, clone AA2
Alternate Names	tubulin, beta polypeptide Tubulin beta-5 chain beta 5-tubulin beta Ib tubulin beta-4 tubulin tubulin beta polypeptide tubulin beta-1 chain tubulin, beta Tubulin beta-5 chain
背景信息	Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each about 55 kDa in size. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) and therefore beta Tubulin should not be used as loading control for these tissues.

产品信息
格式	Purified
控制	Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
演示	Purified mouse monoclonal IgG1 in buffer containing PBS with 0.05% sodium azide and 30% glycerol.

应用
应用	This Anti-β-Tubulin Antibody, clone AA2 is validated for use in WB for the detection of β-Tubulin.
主要应用	Western Blotting

生物信息
免疫原品种	Purified bovine brain tubulin. The epitope recognized by this antibody has been mapped to amino acids 412-430.
表位	The epitope has been mapped to amino acids 412-430
克隆	AA2
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	This antibody recognizes β-Tubulin isotypes I, II, III, IVa, and IVb of ~50 kDa. No cross-reactivity with alpha or gamma tubulin.
同种型	IgG1
品种反应性	BovineHumanRatMouse
Species Reactivity Note	Proven to react with human, rat, bovine, and mouse. Predicted to have broad species cross-reactivity based on sequence homology.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_178014
基因符号	Tubb5 M40 MGC117247 MGC16435 OK/SW-cl.56 OTTHUMP00000029069 TUBB1 TUBB5
纯化方法	Protein G purfied
UniProt编号	P07437
UniProt汇总	FUNCTION: SwissProt: P07437 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. SIZE: 444 amino acids; 49671 Da SUBUNIT: Dimer of alpha and beta chains. TISSUE SPECIFICITY: Ubiquitously expressed with highest levels in spleen, thymus and immature brain. DOMAIN: SwissProt: P07437 The highly acidic C-terminal region may bind cations such as calcium. SIMILARITY: Belongs to the tubulin family.

产品使用声明
质量保证	Routinely evaluated by western blot on RIPA lysates from 3T3/A31, PC-12, Jurkat, or A431 cells. Western Blot Analysis: 0.1-1.0 µg/mL of this lot detected β-Tubulin in RIPA lysates from 3T3/A31, PC-12, Jurkat, and A431 cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage