- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M WB Rb Purified Polyclonal Antibody 描述 产品目录编号 07-189 品牌系列 Upstate
商名 - Upstate
描述 Anti-Notch 4 Antibody 产品信息 格式 Purified 演示 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, and 0.05% sodium azide. 应用 应用 Anti-Notch 4 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Notch 4 & has been validated in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 His-tag fusion protein containing the carboxy-terminus of murine Notch 4 宿主 Rabbit 特异性 truncated, intracellular domain of Int3/Notch 4, possibly full-length Notch 4; does not cross-react with Notch 1, 2 or 3 同种型 IgG 品种反应性 Mouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_004557
Entrez基因汇总 This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. This gene may be associated with susceptibility to schizophrenia in a small portion of cases. An alternative splice variant has been described but its biological nature has not been determined. 基因符号 - FLJ16302
- NOTCH4
- INT3
- MGC74442
- OTTHUMP00000062678
- NOTCH3
- hNotch4
- notch4
纯化方法 Protein A chromatography UniProt编号 - Q99466
UniProt汇总 FUNCTION: SwissProt: Q99466 # Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system (By similarity).
SIZE: 2003 amino acids; 209622 Da
SUBUNIT: Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds (By similarity). Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH4.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Notch 4 intracellular domain: Nucleus. Note=Following proteolytical processing NICD is translocated to the nucleus.
TISSUE SPECIFICITY: Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.
PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C- terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity). & Phosphorylated (By similarity).
SIMILARITY: SwissProt: Q99466 ## Belongs to the NOTCH family. & Contains 5 ANK repeats. & Contains 28 EGF-like domains. & Contains 3 LNR (Lin/Notch) repeats.产品使用声明 质量保证 routinely evaluated by immunoblot on lysates from 293 cells transfected with the intracellular domain of murine Notch 4 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C for up to 12 months from date of receipt. 包装信息 数量 200 µg
上一件merck millipore产品：merck millipore,默克密理博,494127,Ghrelin, Rat, Mouse, Synthetic - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,23-058M,Smurf1, active
相关产品
merck millipore,默克密理博,AP502B,Goat Anti-Human Ig κ chain Antibody, biotin conjugate, Species Adsorbed
![merck millipore,默克密理博,115230,3-[4-(2-羟乙基)-1-哌肼基]-丙烷磺酸](/e/upload/m_pic/MM_watermark%5BMM_watermark-ALL%5D.jpg "merck millipore,默克密理博,115230,3-[4-(2-羟乙基)-1-哌肼基]-丙烷磺酸")
merck millipore,默克密理博,115230,3-[4-(2-羟乙基)-1-哌肼基]-丙烷磺酸
merck millipore,默克密理博,06-500,Anti-p130 Cas Antibody
merck millipore,默克密理博,07-823,Anti-phospho eIF4E (Ser209) Antibody
merck millipore,默克密理博,494127,Ghrelin, Rat, Mouse, Synthetic - Calbiochem
merck millipore,默克密理博,23-058M,Smurf1, active
merck millipore,默克密理博,238590,Cucurbitacin I, Cucumis sativus L. - CAS 117793 - Calbiochem
merck millipore,默克密理博,559304,Anti-SAPK/JNK Rabbit pAb
merck millipore,默克密理博,SVHV01015,Sterivex-HV 过滤器，0.45 µm，PVDF，射线灭菌
