- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB Rb Serum Polyclonal Antibody 描述 产品目录编号 07-1800 描述 Anti-eIF4G Antibody Alternate Names - eukaryotic translation initiation factor 4 gamma, 1
- eIF-4-gamma 1
- eIF-4G 1
背景信息 eIF4F is a translation initiation factor containing three subunits: eIF4A, eIF4E, and eIF4G. This complex is a key component in promoting ribosomal binding to the mRNA. eIF4G interacts with both eIF4A and eIF4E though its carboxy and amino terminal domains, respectively. eIF4E binds to the cap structure of the mRNA, while the ATP-dependent RNA helicase eIF4A unwinds the secondary structure of the 5’ UTR. eIF4G plays a critical role in coordinating the assembly of the eukaryotic initiation factors. It acts as a bridge to bind the cap-bound eIF4E, eIF4AI&II, the eIF3 complex, PABP1, and the kinase Mnk1 (Lejeune, F., et al., 2004) in a large eukaryotic initiation factor complex 产品信息 格式 Serum 演示 Rabbit polyclonal serum with 0.05% NaN3. 应用 应用 Anti-eIF4G Antibody is an antibody against eIF4G for use in WB & IF. 主要应用 - Western Blotting
生物信息 免疫原品种 Synthetic peptide corresponding to N-terminal region of human eIF4G. 表位 N-terminus 宿主 Rabbit 特异性 This antibody detects eIF4G at its N-terminal region. 品种反应性 MouseHuman Species Reactivity Note Human and Mouse. Not tested on other species. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_937884
Entrez基因汇总 The protein encoded by this gene is a component of the protein complex EIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure, and recruitment of mRNA to the ribosome. Alternative splicing results in five transcript variants encoding four distinct isoforms. [provided by RefSeq] 基因符号 - p220
- EIF4-gamma
- EIF4F
- EIF4G
- EIF4GI
- eIF-4G1
UniProt编号 - Q04637
UniProt汇总 FUNCTION: Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome.
SUBUNIT STRUCTURE: eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis.
PTM: Phosphorylated at multiple sites in vivo.
Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.
SEQUENCE SIMILARITIES: Belongs to the eIF4G family.
Contains 1 MI domain.
Contains 1 MIF4G domain.
Contains 1 W2 domain.
SEQUENCE CAUTION: The sequence BAA02185.1 differs from that shown. Reason: Frameshift at several positions.产品使用声明 质量保证 Evaluated by western blot on HeLa cytoplasmic extract.
Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect eIF4G in HeLa cytoplasmic extract and lysates from 293T and S10 cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,AG378,P2Y2
下一件merck millipore产品：merck millipore,默克密理博,341274,Factor H, Human
相关产品
merck millipore,默克密理博,529584,PTK/PI 3-K/mTOR Inhibitor, PP121 - CAS 1092788-83-4 - Calbiochem
merck millipore,默克密理博,566320,Sirtinol - CAS 410536-97-9 - Calbiochem
merck millipore,默克密理博,AB5873,Anti-Catechol O-methyltransferase Antibody
merck millipore,默克密理博,ABT59,Anti-Pericentrin Antibody
merck millipore,默克密理博,AG378,P2Y2
merck millipore,默克密理博,341274,Factor H, Human
merck millipore,默克密理博,SCHVU01RE,Stericup-HV，0.45 µm，PVDF，150 mL，射线灭菌
merck millipore,默克密理博,MABD169,Anti-RUNX1 Antibody, clone 5A1
merck millipore,默克密理博,MAB425,Anti-Carcinoembryonic Antigen Antibody, clone 1105
merck millipore,默克密理博,0500-2260,Guava Flow Cell (HT Systems)