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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M IHC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-158 商名 - Upstate
描述 Anti-KDR/Flk-1/VEGFR2 Antibody Alternate Names - CD309
- Vascular endothelial growth factor receptor 2
- VEGFR-2
- Fetal liver kinase 1
- FLK-1
- Kinase insert domain receptor
- Protein-tyrosine kinase receptor flk-1
背景信息 The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase. 产品信息 格式 Affinity Purified 控制 - PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This KDR/Flk-1/VEGFR2 antibody is validated for use in WB & IHC for the detection of the KDR/Flk-1/VEGFR2 protein. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: 1:100 dilution from a representative lot detected KDR/Flk-1/VEGFR2 in mouse embryo vascular endothelium tissue. 生物信息 免疫原品种 GST-tagged recombinant protein corresponding to mouse KDR/Flk-1/VEGFR2 at the cytoplasmic domain. 表位 Cytoplasmic domain 宿主 Rabbit 特异性 This antibody recognizes KDR/Flk-1/VEGFR2 at the cytoplasmic domain. 品种反应性 Mouse Species Reactivity Note Demonstrated to react with Mouse. Expected to react with Rat based on a 99% sequence homology and Human based on 92% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_002244
基因符号 - CD309
- KDR
- VEGFR-2
- VEGFR2
- FLK1
- VEGFR
纯化方法 Affinity purified UniProt编号 - P35968
UniProt汇总 FUNCTION: Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
SIZE: 1356 amino acids; 151527 Da
SUBUNIT: Interacts with SHB upon VEGF activation. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by Western Blot in PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF.
Western Blot Analysis: 1:1,000 dilution of this antibody detected KDR/Flk-1/VEGFR2 on 10 µg of PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 200 µL
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