merck millipore,默克密理博,07-1570-S,Anti-monomethyl Histone H4 (Lys20) Antibody, Trial Size

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merck millipore,默克密理博,07-1570-S,Anti-monomethyl Histone H4 (Lys20) Antibody, Trial Size
产品名称：Anti-monomethyl Histone H4 (Lys20) Antibody, Trial Size
产品型号：07-1570-S
Anti-monomethyl Histone H4 (Lys20) Antibody, Trial Size is a rabbit polyclonal antibody for detection of monomethyl Histone H4 (Lys20) also known as H4K20me1, Histone H4 (mono methyl K20), H4 histone More>>

merck millipore,默克密理博,07-1570-S,Anti-monomethyl Histone H4 (Lys20) Antibody, Trial Size

产品介绍

merck millipore,默克密理博,07-1570-S,Anti-monomethyl Histone H4 (Lys20) Antibody, Trial Size

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	ChIP, WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	07-1570-S
商名	Upstate
描述	Anti-monomethyl Histone H4 (Lys20) Antibody, Trial Size
Alternate Names	histone cluster 1, H4a H4K20me1 Histone H4 (mono methyl K20) H4 histone family, member A histone 1, H4a
背景信息	Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histones modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). Methylation of histone H4 at lysine 20 has been implicated in transcriptional activation, gene silencing, heterochromatin formation, mitosis, and DNA repair. Histone H4 is progressively methylated at Lys20 during the G(2), M, and G(1) phases of the cell cycle, and methylation of H4Lys20 is a marker for heterochromatin. In mammals, the female X chromosome is coated by the Xist RNA, which is critical for silencing, and is marked mainly by methylation of H3Lys9, H3Lys27 and H4Lys20.

产品信息
格式	Affinity Purified
控制	HeLa cell acid extracts.
演示	Purified from serum by affinity chromatography. Supplied at 1 mg/ml in 0.1M Tris-Glycine (pH7.4), 15mM NaCl, 0.05% NaN3.

应用
应用	Anti-monomethyl Histone H4 (Lys20) Antibody, Trial Size is a rabbit polyclonal antibody for detection of monomethyl Histone H4 (Lys20) also known as H4K20me1, Histone H4 (mono methyl K20), H4 histone family member A & has been validated in WB.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting
应用说明	Chromatin Immunoprecipitation: Sonicated chromatin prepared from HeLa cells (2 X 10⁶ cell equivalents per IP) was subjected to chromatin immunoprecipitation using 4 μg of either a negative control antibody or Anti-Monomethyl-Histone H4 (Lys20) antibody and the Magna ChIP A Kit (Cat. #17-610). Successful immunoprecipitation of monomethyl-histone H4 (Lys20)-associated DNA fragments was verified by qPCR using GAPDH coding region ChIP Primers versus Control Primers corresponding to the GAPDH promoter (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin, with immunoprecipitated DNA from negative control antibody shown as (-) and monomethyl-histone H4 (Lys20) shown as (+). Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. Western Blot Analysis: 0.1 μg/mL of proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures) Peptide Blocking Assay: 40 μg of histone H4 peptide containing monomethyl Lys20 abolished detection of histone H4 by anti-monomethyl-Histone H4 (Lys20) in immunoblot analysis of acid extracts of HeLa cells (Please see figures).

生物信息
免疫原品种	KLH-conjugated, linear synthetic peptide corresponding to amino acids 15-24 (AKRHR[me1K]VLRD-C) of human Histone H4.
表位	Monomethyl Lys20
宿主	Rabbit
特异性	Recognizes monomethylated Histone H4 (Lys20), Mr 11 kDa.
品种反应性	Human
Species Reactivity Note	Human. Based on sequence homology, broad species cross-reactivity is expected
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_003538.3
Entrez基因汇总	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]
基因符号	HIST2H4 H4/A H4/B H4/C H4/D H4/E H4/G H4/H H4/I H4/J H4/K H4/M H4/N H4/O H4/a H4F2 H4FA H4FB H4FC H4FD H4FE H4FG H4FH H4FI H4FJ H4FK H4FM H4FN H4FO
修改	Methylation
纯化方法	Affinity chromatography
UniProt编号	P62805
UniProt汇总	FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA SUBCELLULAR LOCATION: Nucleus. PTM: Acetylation at Lys-6, Lys-9, Lys-13 and Lys-17 occurs in coding regions of the genome but not in heterochromatin. PTM: Citrullination at Arg-4 by PADI4 impairs methylation. PTM: Monomethylation at Arg-4 by PRMT1 favors acetylation at Lys-9 and Lys-13. Demethylation is performed by JMJD6. PTM: Monomethylated, dimethylated or trimethylated at Lys-21. Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. PTM: Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. PTM: Sumoylated, which is associated with transcriptional repression. SIMILARITY: Belongs to the histone H4 family.

产品使用声明
质量保证	Routinely evaluated by western blot analysis
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.